{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":65,"searchCount":0},"additional":{"submitter":["Kumar A"],"funding":["NIA NIH HHS"],"pagination":["254-63"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3249674"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["153(1)"],"pubmed_abstract":["In the bone marrow cavity, adipocyte numbers increase, whereas osteoblast progenitor numbers decrease with aging. Because adipocytes and osteoblasts share a common progenitor, it is possible that this shift is due to an increase in adipocyte-lineage cells at the expense of osteoblast-lineage commitment. Estrogens inhibit adipocyte differentiation, and in both men and women, circulating estrogens correlate with bone loss with aging. In bone cells, estrogens stimulate expression of TGF-? and suppress mesenchymal cell adipogenesis. Using a tripotential mesenchymal cell line, we have examined whether estradiol suppression of adipocyte differentiation is due to stimulation of TGF-? and the mechanism by which TGF-? suppresses adipogenesis. We observed that estradiol-mediated suppression of adipogenic gene expression required at least 48 h treatment. TGF-? expression increased within 24 h of estradiol treatment, and TGF-? inhibition reversed estradiol influences on adipogenesis and adipocyte gene expression. Connective tissue growth factor (CTGF) mediates TGF-? suppression of adipogenesis in mouse 3T3-L1 cells. CTGF expression was induced within 24 h of TGF-? treatment, whereas estradiol-mediated induction required 48 h treatment. Moreover, estradiol-mediated induction of CTGF was abrogated by TGF-? inhibition. These data support that estradiol effects on adipogenesis involves TGF-? induction, which then induces CTGF to suppress adipogenesis."],"journal":["Endocrinology"],"pubmed_title":["TGF-? mediates suppression of adipogenesis by estradiol through connective tissue growth factor induction."],"pmcid":["PMC3249674"],"funding_grant_id":["R01 AG028936","P01 AG004875"],"pubmed_authors":["Syed F","Clifton K","Ruan M","Khosla S","Oursler MJ","Kumar A"],"view_count":["65"],"additional_accession":[]},"is_claimable":false,"name":"TGF-? mediates suppression of adipogenesis by estradiol through connective tissue growth factor induction.","description":"In the bone marrow cavity, adipocyte numbers increase, whereas osteoblast progenitor numbers decrease with aging. Because adipocytes and osteoblasts share a common progenitor, it is possible that this shift is due to an increase in adipocyte-lineage cells at the expense of osteoblast-lineage commitment. Estrogens inhibit adipocyte differentiation, and in both men and women, circulating estrogens correlate with bone loss with aging. In bone cells, estrogens stimulate expression of TGF-? and suppress mesenchymal cell adipogenesis. Using a tripotential mesenchymal cell line, we have examined whether estradiol suppression of adipocyte differentiation is due to stimulation of TGF-? and the mechanism by which TGF-? suppresses adipogenesis. We observed that estradiol-mediated suppression of adipogenic gene expression required at least 48 h treatment. TGF-? expression increased within 24 h of estradiol treatment, and TGF-? inhibition reversed estradiol influences on adipogenesis and adipocyte gene expression. Connective tissue growth factor (CTGF) mediates TGF-? suppression of adipogenesis in mouse 3T3-L1 cells. CTGF expression was induced within 24 h of TGF-? treatment, whereas estradiol-mediated induction required 48 h treatment. Moreover, estradiol-mediated induction of CTGF was abrogated by TGF-? inhibition. These data support that estradiol effects on adipogenesis involves TGF-? induction, which then induces CTGF to suppress adipogenesis.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jan","modification":"2020-10-01T07:21:04Z","creation":"2019-03-27T00:47:33Z"},"accession":"S-EPMC3249674","cross_references":{"pubmed":["22067314"],"doi":["10.1210/en.2011-1169"]}}