biostudies-literatureZhang HNCRR NIH HHS2532-44https://www.ebi.ac.uk/biostudies/studies/S-EPMC3253737biostudies-literatureUnknown74(12)Fourteen new withanolides, 1-14, named withalongolides A-N, respectively, were isolated from the aerial parts of Physalis longifolia together with eight known compounds (15-22). The structures of compounds 1-14 were elucidated through spectroscopic techniques and chemical methods. In addition, the structures of withanolides 1, 2, 3, and 6 were confirmed by X-ray crystallographic analysis. Using a MTS viability assay, eight withanolides (1, 2, 3, 7, 8, 15, 16, and 19) and four acetylated derivatives (1a, 1b, 2a, and 2b) showed potent cytotoxicity against human head and neck squamous cell carcinoma (JMAR and MDA-1986), melanoma (B16F10 and SKMEL-28), and normal fetal fibroblast (MRC-5) cells with IC₅₀ values in the range between 0.067 and 9.3 μM.Journal of natural productsCytotoxic withanolide constituents of Physalis longifolia.PMC3253737P20 RR015563P20 RR015563-10Day VWSamadi AKGallagher RJTimmermann BNAraya JJZhang HTong XGollapudi RCohen MSKindscher KfalseCytotoxic withanolide constituents of Physalis longifolia.Fourteen new withanolides, 1-14, named withalongolides A-N, respectively, were isolated from the aerial parts of Physalis longifolia together with eight known compounds (15-22). The structures of compounds 1-14 were elucidated through spectroscopic techniques and chemical methods. In addition, the structures of withanolides 1, 2, 3, and 6 were confirmed by X-ray crystallographic analysis. Using a MTS viability assay, eight withanolides (1, 2, 3, 7, 8, 15, 16, and 19) and four acetylated derivatives (1a, 1b, 2a, and 2b) showed potent cytotoxicity against human head and neck squamous cell carcinoma (JMAR and MDA-1986), melanoma (B16F10 and SKMEL-28), and normal fetal fibroblast (MRC-5) cells with IC₅₀ values in the range between 0.067 and 9.3 μM.2011-01-01T00:00:00Z2011 Dec2024-11-10T00:38:49.477Z2019-03-27T00:47:43ZS-EPMC32537372209861110.1021/np200635r