{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Falk A"],"funding":["Medical Research Council","European Commission FP7","The Brain Tumour Charity","Wellcome Trust"],"pagination":["e29597"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3260177"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; "],"journal":["PloS one"],"pubmed_title":["Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons."],"pmcid":["PMC3260177"],"funding_grant_id":["8/105","G1001028","MC_PC_12009","G0800784B","FP7_222943","G0800784","G1100526","079249"],"pubmed_authors":["Ladewig J","Takashima Y","Falk A","Kesavan J","Smith A","Wiskow O","Brustle O","Trotter M","Pollard S","Koch P","Alexander M","Tailor J"],"additional_accession":[]},"is_claimable":false,"name":"Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.","description":"Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; ","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2025-04-26T16:48:37.537Z","creation":"2019-03-26T23:08:25Z"},"accession":"S-EPMC3260177","cross_references":{"pubmed":["22272239"],"doi":["10.1371/journal.pone.0029597"]}}