biostudies-literatureFalk AMedical Research CouncilEuropean Commission FP7The Brain Tumour CharityWellcome Truste29597https://www.ebi.ac.uk/biostudies/studies/S-EPMC3260177biostudies-literatureUnknown7(1)Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; PloS oneCapture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.PMC32601778/105G1001028MC_PC_12009G0800784BFP7_222943G0800784G1100526079249Ladewig JTakashima YFalk AKesavan JSmith AWiskow OBrustle OTrotter MPollard SKoch PAlexander MTailor JfalseCapture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; 2012-01-01T00:00:00Z20122025-04-26T16:48:37.537Z2019-03-26T23:08:25ZS-EPMC32601772227223910.1371/journal.pone.0029597