{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Musiyenko A"],"funding":["NIAID NIH HHS"],"pagination":["745-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3263383"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["85(7)"],"pubmed_abstract":["Stable RNA interference (RNAi) is commonly achieved by recombinant expression of short hairpin RNA (shRNA). To generate virus-resistant cell lines, we cloned a shRNA cassette against the phosphoprotein gene of respiratory syncytial virus (RSV) into a polIII-driven plasmid vector. Analysis of individual stable transfectants showed a spectrum of RSV resistance correlating with the levels of shRNA expressed from different chromosomal locations. Interestingly, resistance in a minority of clones was due to mono-allelic disruption of the cellular gene for vasodilator-stimulated phosphoprotein (VASP). Thus, pure clones of chromosomally integrated DNA-directed RNAi can exhibit gene disruption phenotypes resembling but unrelated to RNAi."],"journal":["Journal of molecular medicine (Berlin, Germany)"],"pubmed_title":["RNAi-dependent and -independent antiviral phenotypes of chromosomally integrated shRNA clones: role of VASP in respiratory syncytial virus growth."],"pmcid":["PMC3263383"],"funding_grant_id":["R01 AI059267","AI059267","R01 AI059267-03","R01 AI059267-04","R01 AI059267-05","R01 AI059267-06"],"pubmed_authors":["Barik S","Musiyenko A","Bitko V"],"additional_accession":[]},"is_claimable":false,"name":"RNAi-dependent and -independent antiviral phenotypes of chromosomally integrated shRNA clones: role of VASP in respiratory syncytial virus growth.","description":"Stable RNA interference (RNAi) is commonly achieved by recombinant expression of short hairpin RNA (shRNA). To generate virus-resistant cell lines, we cloned a shRNA cassette against the phosphoprotein gene of respiratory syncytial virus (RSV) into a polIII-driven plasmid vector. Analysis of individual stable transfectants showed a spectrum of RSV resistance correlating with the levels of shRNA expressed from different chromosomal locations. Interestingly, resistance in a minority of clones was due to mono-allelic disruption of the cellular gene for vasodilator-stimulated phosphoprotein (VASP). Thus, pure clones of chromosomally integrated DNA-directed RNAi can exhibit gene disruption phenotypes resembling but unrelated to RNAi.","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Jul","modification":"2025-04-29T09:47:58.169Z","creation":"2019-06-05T17:00:33Z"},"accession":"S-EPMC3263383","cross_references":{"pubmed":["17351763"],"doi":["10.1007/s00109-007-0179-0"]}}