{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rolfe MD"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["686-701"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3264077"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["194(3)"],"pubmed_abstract":["Lag phase represents the earliest and most poorly understood stage of the bacterial growth cycle. We developed a reproducible experimental system and conducted functional genomic and physiological analyses of a 2-h lag phase in Salmonella enterica serovar Typhimurium. Adaptation began within 4 min of inoculation into fresh LB medium with the transient expression of genes involved in phosphate uptake. The main lag-phase transcriptional program initiated at 20 min with the upregulation of 945 genes encoding processes such as transcription, translation, iron-sulfur protein assembly, nucleotide metabolism, LPS biosynthesis, and aerobic respiration. ChIP-chip revealed that RNA polymerase was not \"poised\" upstream of the bacterial genes that are rapidly induced at the beginning of lag phase, sug"],"journal":["Journal of bacteriology"],"pubmed_title":["Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation."],"pmcid":["PMC3264077"],"funding_grant_id":["BBS/S/M/2006/13059","BBS/E/F/00044406","BBS/S/N/2003/10520","BBS/E/F/00044401"],"pubmed_authors":["Pin C","Rolfe MD","Thompson A","Betts RP","Rice CJ","Baranyi J","Alston M","Cameron AD","Hinton JC","Lucchini S","Stringer MF","Peck MW"],"additional_accession":[]},"is_claimable":false,"name":"Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation.","description":"Lag phase represents the earliest and most poorly understood stage of the bacterial growth cycle. We developed a reproducible experimental system and conducted functional genomic and physiological analyses of a 2-h lag phase in Salmonella enterica serovar Typhimurium. Adaptation began within 4 min of inoculation into fresh LB medium with the transient expression of genes involved in phosphate uptake. The main lag-phase transcriptional program initiated at 20 min with the upregulation of 945 genes encoding processes such as transcription, translation, iron-sulfur protein assembly, nucleotide metabolism, LPS biosynthesis, and aerobic respiration. ChIP-chip revealed that RNA polymerase was not \"poised\" upstream of the bacterial genes that are rapidly induced at the beginning of lag phase, sug","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Feb","modification":"2026-05-01T01:07:45.599Z","creation":"2026-04-07T16:28:22.884Z"},"accession":"S-EPMC3264077","cross_references":{"pubmed":["22139505"],"doi":["10.1128/jb.06112-11","10.1128/JB.06112-11"]}}