{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Pazos-Navarro M"],"pubmed_abstract":["<h4>Background</h4>Bituminaria bituminosa is a perennial legume species from the Canary Islands and Mediterranean region that has potential as a drought-tolerant pasture species and as a source of pharmaceutical compounds. Three botanical varieties have previously been identified in this species: albomarginata, bituminosa and crassiuscula. B. bituminosa can be considered a genomic 'orphan' species with very few genomic resources available. New DNA sequencing technologies provide an opportunity to develop high quality molecular markers for such orphan species.<h4>Results</h4>432,306 mRNA molecules were sampled from a leaf transcriptome of a single B. bituminosa plant using Roche 454 pyrosequencing, resulting in an average read length of 345 bp (149.1 Mbp in total). Sequences were assembled "],"journal":["BMC genetics"],"pagination":["104"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3265443"],"repository":["biostudies-literature"],"pubmed_title":["Next generation DNA sequencing technology delivers valuable genetic markers for the genomic orphan legume species, Bituminaria bituminosa."],"pmcid":["PMC3265443"],"pubmed_authors":["Hanson K","Teakle N","Real D","Nelson MN","Pazos-Navarro M","Dabauza M","Correal E"],"additional_accession":[]},"is_claimable":false,"name":"Next generation DNA sequencing technology delivers valuable genetic markers for the genomic orphan legume species, Bituminaria bituminosa.","description":"<h4>Background</h4>Bituminaria bituminosa is a perennial legume species from the Canary Islands and Mediterranean region that has potential as a drought-tolerant pasture species and as a source of pharmaceutical compounds. Three botanical varieties have previously been identified in this species: albomarginata, bituminosa and crassiuscula. B. bituminosa can be considered a genomic 'orphan' species with very few genomic resources available. New DNA sequencing technologies provide an opportunity to develop high quality molecular markers for such orphan species.<h4>Results</h4>432,306 mRNA molecules were sampled from a leaf transcriptome of a single B. bituminosa plant using Roche 454 pyrosequencing, resulting in an average read length of 345 bp (149.1 Mbp in total). Sequences were assembled ","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Dec","modification":"2026-04-07T18:01:11.494Z","creation":"2026-04-07T16:20:55.274Z"},"accession":"S-EPMC3265443","cross_references":{"pubmed":["22171578"],"doi":["10.1186/1471-2156-12-104"]}}