biostudies-literatureTianero MDNIGMS NIH HHS418-25https://www.ebi.ac.uk/biostudies/studies/S-EPMC3268757biostudies-literatureUnknown134(1)The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in Escherichia coli . These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.Journal of the American Chemical SocietyRibosomal route to small-molecule diversity.PMC3268757R01 GM097206R01 GM071425-05R01 GM071425-04R01 GM071425-03R01 GM071425-01A1GM097206R01 GM071425-02GM071425-S1GM071425R01 GM071425Schmidt EWDonia MSYoung TSTianero MDSchultz PGfalseRibosomal route to small-molecule diversity.The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in Escherichia coli . These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.2012-01-01T00:00:00Z2012 Jan2024-12-04T03:07:58.539Z2019-03-26T23:29:44ZS-EPMC32687572210759310.1021/ja208278k