{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["109(3)"],"submitter":["Quirin S"],"pubmed_abstract":["Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing only a sparse, random set of molecules to emit light at any given time and subsequently localizing each molecule with great precision. Recently, such techniques have been extended to three dimensions, opening up unprecedented possibilities to explore the structure and function of cells. Interestingly, proper engineering of the three-dimensional (3D) point spread function (PSF) through additional optics has been demonstrated to theoretica"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["675-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3271897"],"repository":["biostudies-literature"],"pubmed_title":["Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions."],"pmcid":["PMC3271897"],"pubmed_authors":["Pavani SR","Piestun R","Quirin S"],"additional_accession":[]},"is_claimable":false,"name":"Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions.","description":"Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing only a sparse, random set of molecules to emit light at any given time and subsequently localizing each molecule with great precision. Recently, such techniques have been extended to three dimensions, opening up unprecedented possibilities to explore the structure and function of cells. Interestingly, proper engineering of the three-dimensional (3D) point spread function (PSF) through additional optics has been demonstrated to theoretica","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jan","modification":"2025-04-19T08:03:59.11Z","creation":"2019-03-27T00:48:43Z"},"accession":"S-EPMC3271897","cross_references":{"pubmed":["22210112"],"doi":["10.1073/pnas.1109011108"]}}