biostudies-literatureUnknown53(3)Dodes Traian MMLateral organization of biological membranes is frequently studied using fluorescence microscopy. One of the most widely used probes for these studies is 2-dimethylamino-6-lauroylnaphthalene (laurdan). The fluorescence of this probe is sensitive to the environment polarity, and thus laurdan reports the local penetration of water when inserted in membranes. Unfortunately, this probe can only be used under two-photon excitation due to its low photostability. This is a very important limitation, because there are not too many laboratories with capability for two-photon microscopy. In this work, we explored the performance of 6-dodecanoyl-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (C-laurdan), a carboxyl-modified version of laurdan, for imaging biological membranes using a conventional conJournal of lipid research609-616https://www.ebi.ac.uk/biostudies/studies/S-EPMC3276485biostudies-literatureImaging lipid lateral organization in membranes with C-laurdan in a confocal microscope.PMC3276485Levi VDodes Traian MMFlecha FLGfalseImaging lipid lateral organization in membranes with C-laurdan in a confocal microscope.Lateral organization of biological membranes is frequently studied using fluorescence microscopy. One of the most widely used probes for these studies is 2-dimethylamino-6-lauroylnaphthalene (laurdan). The fluorescence of this probe is sensitive to the environment polarity, and thus laurdan reports the local penetration of water when inserted in membranes. Unfortunately, this probe can only be used under two-photon excitation due to its low photostability. This is a very important limitation, because there are not too many laboratories with capability for two-photon microscopy. In this work, we explored the performance of 6-dodecanoyl-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (C-laurdan), a carboxyl-modified version of laurdan, for imaging biological membranes using a conventional con2012-01-01T00:00:00Z2012 Mar2025-04-26T07:40:42.334Z2019-03-27T00:48:58ZS-EPMC32764852218475710.1194/jlr.d02131110.1194/jlr.D021311