{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou W"],"funding":["NCRR NIH HHS"],"pagination":["e31997"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3278458"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(2)"],"pubmed_abstract":["We have recently developed a so-called genomic engineering approach that allows for directed, efficient and versatile modifications of Drosophila genome by combining the homologous recombination (HR)-based gene targeting with site-specific DNA integration. In genomic engineering and several similar approaches, a \"founder\" knock-out line must be generated first through HR-based gene targeting, which can still be a potentially time and resource intensive process. To significantly improve the efficiency and success rate of HR-based gene targeting in Drosophila, we have generated a new dual-selection marker termed W::Neo, which is a direct fusion between proteins of eye color marker White (W) and neomycin resistance (Neo). In HR-based gene targeting experiments, mutants carrying W::Neo as the "],"journal":["PloS one"],"pubmed_title":["W::Neo: a novel dual-selection marker for high efficiency gene targeting in Drosophila."],"pmcid":["PMC3278458"],"funding_grant_id":["R21 RR024869","R21RR024869"],"pubmed_authors":["Huang J","Hong Y","Watson AM","Zhou W"],"additional_accession":[]},"is_claimable":false,"name":"W::Neo: a novel dual-selection marker for high efficiency gene targeting in Drosophila.","description":"We have recently developed a so-called genomic engineering approach that allows for directed, efficient and versatile modifications of Drosophila genome by combining the homologous recombination (HR)-based gene targeting with site-specific DNA integration. In genomic engineering and several similar approaches, a \"founder\" knock-out line must be generated first through HR-based gene targeting, which can still be a potentially time and resource intensive process. To significantly improve the efficiency and success rate of HR-based gene targeting in Drosophila, we have generated a new dual-selection marker termed W::Neo, which is a direct fusion between proteins of eye color marker White (W) and neomycin resistance (Neo). In HR-based gene targeting experiments, mutants carrying W::Neo as the ","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2025-04-22T07:04:28.19Z","creation":"2019-03-26T23:12:37Z"},"accession":"S-EPMC3278458","cross_references":{"pubmed":["22348139"],"doi":["10.1371/journal.pone.0031997"]}}