biostudies-literatureZhou WNCRR NIH HHSe31997https://www.ebi.ac.uk/biostudies/studies/S-EPMC3278458biostudies-literatureUnknown7(2)We have recently developed a so-called genomic engineering approach that allows for directed, efficient and versatile modifications of Drosophila genome by combining the homologous recombination (HR)-based gene targeting with site-specific DNA integration. In genomic engineering and several similar approaches, a "founder" knock-out line must be generated first through HR-based gene targeting, which can still be a potentially time and resource intensive process. To significantly improve the efficiency and success rate of HR-based gene targeting in Drosophila, we have generated a new dual-selection marker termed W::Neo, which is a direct fusion between proteins of eye color marker White (W) and neomycin resistance (Neo). In HR-based gene targeting experiments, mutants carrying W::Neo as the PloS oneW::Neo: a novel dual-selection marker for high efficiency gene targeting in Drosophila.PMC3278458R21 RR024869R21RR024869Huang JHong YWatson AMZhou WfalseW::Neo: a novel dual-selection marker for high efficiency gene targeting in Drosophila.We have recently developed a so-called genomic engineering approach that allows for directed, efficient and versatile modifications of Drosophila genome by combining the homologous recombination (HR)-based gene targeting with site-specific DNA integration. In genomic engineering and several similar approaches, a "founder" knock-out line must be generated first through HR-based gene targeting, which can still be a potentially time and resource intensive process. To significantly improve the efficiency and success rate of HR-based gene targeting in Drosophila, we have generated a new dual-selection marker termed W::Neo, which is a direct fusion between proteins of eye color marker White (W) and neomycin resistance (Neo). In HR-based gene targeting experiments, mutants carrying W::Neo as the 2012-01-01T00:00:00Z20122025-04-22T07:04:28.19Z2019-03-26T23:12:37ZS-EPMC32784582234813910.1371/journal.pone.0031997