{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(2)"],"submitter":["Raza SE"],"pubmed_abstract":["<h4>Background</h4>In recent years, new microscopic imaging techniques have evolved to allow us to visualize several different proteins (or other biomolecules) in a visual field. Analysis of protein co-localization becomes viable because molecules can interact only when they are located close to each other. We present a novel approach to align images in a multi-tag fluorescence image stack. The proposed approach is applicable to multi-tag bioimaging systems which (a) acquire fluorescence images by sequential staining and (b) simultaneously capture a phase contrast image corresponding to each of the fluorescence images. To the best of our knowledge, there is no existing method in the literature, which addresses simultaneous registration of multi-tag bioimages and selection of the reference "],"journal":["PloS one"],"pagination":["e30894"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3280195"],"repository":["biostudies-literature"],"pubmed_title":["RAMTaB: robust alignment of multi-tag bioimages."],"pmcid":["PMC3280195"],"pubmed_authors":["Abouna S","Raza SE","Khan M","Rajpoot NM","Humayun A","Nattkemper TW","Epstein DB"],"additional_accession":[]},"is_claimable":false,"name":"RAMTaB: robust alignment of multi-tag bioimages.","description":"<h4>Background</h4>In recent years, new microscopic imaging techniques have evolved to allow us to visualize several different proteins (or other biomolecules) in a visual field. Analysis of protein co-localization becomes viable because molecules can interact only when they are located close to each other. We present a novel approach to align images in a multi-tag fluorescence image stack. The proposed approach is applicable to multi-tag bioimaging systems which (a) acquire fluorescence images by sequential staining and (b) simultaneously capture a phase contrast image corresponding to each of the fluorescence images. To the best of our knowledge, there is no existing method in the literature, which addresses simultaneous registration of multi-tag bioimages and selection of the reference ","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2025-04-04T21:05:36.807Z","creation":"2019-03-26T23:12:37Z"},"accession":"S-EPMC3280195","cross_references":{"pubmed":["22363510"],"doi":["10.1371/journal.pone.0030894"]}}