biostudies-literatureUnknown50(3)Fernandez-Olmos AClonal isolates identified as various nonfermentative Gram-negative bacilli over a 5-year period from sputum cultures of a 30-year-old cystic fibrosis patient were successfully reidentified as Pandoraea sputorum by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Decreased lung function improved after 1 year of azithromycin and inhaled 7%-hypertonic saline treatment.Journal of clinical microbiology1096-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC3295115biostudies-literatureClinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry.PMC3295115Fernandez-Olmos AGarcia-Castillo MCanton RLamas AMorosini MIGarcia-Garcia LMaiz LfalseClinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry.Clonal isolates identified as various nonfermentative Gram-negative bacilli over a 5-year period from sputum cultures of a 30-year-old cystic fibrosis patient were successfully reidentified as Pandoraea sputorum by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Decreased lung function improved after 1 year of azithromycin and inhaled 7%-hypertonic saline treatment.2012-01-01T00:00:00Z2012 Mar2025-04-19T15:49:28.444Z2019-03-27T00:50:53ZS-EPMC32951152217092210.1128/jcm.05730-1110.1128/JCM.05730-11