{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nakamura J"],"funding":["NCRR NIH HHS","NIEHS NIH HHS"],"pagination":["e33563"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3299801"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(3)"],"pubmed_abstract":["While isogenic DT40 cell lines deficient in DNA repair pathways are a great tool to understand the DNA damage response to genotoxic agents by a comparison of cell toxicity in mutants and parental DT40 cells, no convenient mutation assay for mutagens currently exists for this reverse-genetic system. Here we establish a proaerolysin (PA) selection-based mutation assay in DT40 cells to identify glycosylphosphatidylinositol (GPI)-anchor deficient cells. Using PA, we detected an increase in the number of PA-resistant DT40 cells exposed to MMS for 24 hours followed by a 5-day period of phenotype expression. GPI anchor synthesis is catalyzed by a series of phosphatidylinositol glycan complementation groups (PIGs). The PIG-O gene is on the sex chromosome (Chromosome Z) in chicken cells and is crit"],"journal":["PloS one"],"pubmed_title":["Detection of PIGO-deficient cells using proaerolysin: a valuable tool to investigate mechanisms of mutagenesis in the DT40 cell system."],"pmcid":["PMC3299801"],"funding_grant_id":["P42 ES005948","UL1 RR025747","P30-ES10126","UL1RR025747","P30 ES010126","P42-ES05948"],"pubmed_authors":["Nakamura J","Tian X","Gul H","Bultman SJ","Swenberg JA"],"additional_accession":[]},"is_claimable":false,"name":"Detection of PIGO-deficient cells using proaerolysin: a valuable tool to investigate mechanisms of mutagenesis in the DT40 cell system.","description":"While isogenic DT40 cell lines deficient in DNA repair pathways are a great tool to understand the DNA damage response to genotoxic agents by a comparison of cell toxicity in mutants and parental DT40 cells, no convenient mutation assay for mutagens currently exists for this reverse-genetic system. Here we establish a proaerolysin (PA) selection-based mutation assay in DT40 cells to identify glycosylphosphatidylinositol (GPI)-anchor deficient cells. Using PA, we detected an increase in the number of PA-resistant DT40 cells exposed to MMS for 24 hours followed by a 5-day period of phenotype expression. GPI anchor synthesis is catalyzed by a series of phosphatidylinositol glycan complementation groups (PIGs). The PIG-O gene is on the sex chromosome (Chromosome Z) in chicken cells and is crit","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2026-05-02T08:13:05.968Z","creation":"2026-04-07T17:47:20.782Z"},"accession":"S-EPMC3299801","cross_references":{"pubmed":["22428069"],"doi":["10.1371/journal.pone.0033563"]}}