{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":46,"searchCount":0},"additional":{"submitter":["Chen C"],"funding":["Intramural NIH HHS","NIDDK NIH HHS"],"pagination":["9601-12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3308796"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["287(12)"],"pubmed_abstract":["The roundworm Caenorhabditis elegans is a heme auxotroph that requires the coordinated actions of HRG-1 heme permeases to transport environmental heme into the intestine and HRG-3, a secreted protein, to deliver intestinal heme to other tissues including the embryo. Here we show that heme homeostasis in the extraintestinal hypodermal tissue was facilitated by the transmembrane protein HRG-2. Systemic heme deficiency up-regulated hrg-2 mRNA expression over 200-fold in the main body hypodermal syncytium, hyp 7. HRG-2 is a type I membrane protein that binds heme and localizes to the endoplasmic reticulum and apical plasma membrane. Cytochrome heme profiles are aberrant in HRG-2-deficient worms, a phenotype that was partially suppressed by heme supplementation. A heme-deficient yeast strain, ectopically expressing worm HRG-2, revealed significantly improved growth at submicromolar concentrations of exogenous heme. Taken together, our results implicate HRG-2 as a facilitator of heme utilization in the Caenorhabditis elegans hypodermis and provide a mechanism for the regulation of heme homeostasis in an extraintestinal tissue."],"journal":["The Journal of biological chemistry"],"pubmed_title":["Heme utilization in the Caenorhabditis elegans hypodermal cells is facilitated by heme-responsive gene-2."],"pmcid":["PMC3308796"],"funding_grant_id":["R56 DK032303","R01DK74797","R01 DK074797"],"pubmed_authors":["Krause M","Dailey HA","Samuel TK","Hamza I","Chen C"],"view_count":["46"],"additional_accession":[]},"is_claimable":false,"name":"Heme utilization in the Caenorhabditis elegans hypodermal cells is facilitated by heme-responsive gene-2.","description":"The roundworm Caenorhabditis elegans is a heme auxotroph that requires the coordinated actions of HRG-1 heme permeases to transport environmental heme into the intestine and HRG-3, a secreted protein, to deliver intestinal heme to other tissues including the embryo. Here we show that heme homeostasis in the extraintestinal hypodermal tissue was facilitated by the transmembrane protein HRG-2. Systemic heme deficiency up-regulated hrg-2 mRNA expression over 200-fold in the main body hypodermal syncytium, hyp 7. HRG-2 is a type I membrane protein that binds heme and localizes to the endoplasmic reticulum and apical plasma membrane. Cytochrome heme profiles are aberrant in HRG-2-deficient worms, a phenotype that was partially suppressed by heme supplementation. A heme-deficient yeast strain, ectopically expressing worm HRG-2, revealed significantly improved growth at submicromolar concentrations of exogenous heme. Taken together, our results implicate HRG-2 as a facilitator of heme utilization in the Caenorhabditis elegans hypodermis and provide a mechanism for the regulation of heme homeostasis in an extraintestinal tissue.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Mar","modification":"2021-03-18T08:05:52Z","creation":"2019-03-27T00:51:25Z"},"accession":"S-EPMC3308796","cross_references":{"pubmed":["22303006"],"doi":["10.1074/jbc.m111.307694","10.1074/jbc.M111.307694"]}}