{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["1(3)"],"submitter":["Shivram H"],"pubmed_abstract":["Restriction-like endonuclease (RLE) bearing non-LTR retrotransposons are site-specific elements that integrate into the genome through target primed reverse transcription (TPRT). RLE-bearing elements have been used as a model system for investigating non-LTR retrotransposon integration. R2 elements target a specific site in the 28S rDNA gene. We previously demonstrated that the two major sub-classes of R2 (R2-A and R2-D) target the R2 insertion site in an opposing manner with regard to the pairing of known DNA binding domains and bound sequences-indicating that the A- and D-clades represent independently derived modes of targeting that site. Elements have been discovered that group phylogenetically with R2 but do not target the canonical R2 site. Here we extend our earlier studies to show "],"journal":["Mobile genetic elements"],"pagination":["169-178"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3312299"],"repository":["biostudies-literature"],"pubmed_title":["Targeting novel sites: The N-terminal DNA binding domain of non-LTR retrotransposons is an adaptable module that is implicated in changing site specificities."],"pmcid":["PMC3312299"],"pubmed_authors":["Cawley D","Christensen SM","Shivram H"],"additional_accession":[]},"is_claimable":false,"name":"Targeting novel sites: The N-terminal DNA binding domain of non-LTR retrotransposons is an adaptable module that is implicated in changing site specificities.","description":"Restriction-like endonuclease (RLE) bearing non-LTR retrotransposons are site-specific elements that integrate into the genome through target primed reverse transcription (TPRT). RLE-bearing elements have been used as a model system for investigating non-LTR retrotransposon integration. R2 elements target a specific site in the 28S rDNA gene. We previously demonstrated that the two major sub-classes of R2 (R2-A and R2-D) target the R2 insertion site in an opposing manner with regard to the pairing of known DNA binding domains and bound sequences-indicating that the A- and D-clades represent independently derived modes of targeting that site. Elements have been discovered that group phylogenetically with R2 but do not target the canonical R2 site. Here we extend our earlier studies to show ","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Sep","modification":"2025-04-03T22:54:33.323Z","creation":"2019-03-27T00:51:36Z"},"accession":"S-EPMC3312299","cross_references":{"pubmed":["22479684"],"doi":["10.4161/mge.1.3.18453"]}}