biostudies-literatureUnknown190(4)Henikoff SHoward Hughes Medical InstituteThe "point" centromere of budding yeast is genetically defined by an ≈ 125-bp sequence. Recent fluorescence measurements of kinetochore clusters have suggested that this sequence specifies multiple centromere histone 3 (CenH3) nucleosomes. However, high-resolution mapping demonstrates that there is only one CenH3 nucleosome per centromere, providing biochemical confirmation of the point centromere model.Genetics1575-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC3316665biostudies-literature"Point" centromeres of Saccharomyces harbor single centromere-specific nucleosomes.PMC3316665Henikoff SHenikoff JGfalse"Point" centromeres of Saccharomyces harbor single centromere-specific nucleosomes.The "point" centromere of budding yeast is genetically defined by an ≈ 125-bp sequence. Recent fluorescence measurements of kinetochore clusters have suggested that this sequence specifies multiple centromere histone 3 (CenH3) nucleosomes. However, high-resolution mapping demonstrates that there is only one CenH3 nucleosome per centromere, providing biochemical confirmation of the point centromere model.2012-01-01T00:00:00Z2012 Apr2025-04-25T23:16:05.827Z2019-06-05T17:11:34ZS-EPMC33166652223485610.1534/genetics.111.137711