biostudies-literatureUnknown287(13)Okuda-Ashitaka E4-Nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) is a molecule of physiologically unknown function, although it is predominantly expressed in the brain, spinal cord, liver, and kidney. We identified NIPSNAP1 as a protein that interacts with the neuropeptide nocistatin (NST) from synaptosomal membranes of mouse spinal cord using high-performance affinity latex beads. NST, which is produced from the same precursor protein as an opioid-like neuropeptide nociceptin/orphanin FQ (N/OFQ), has opposite effects on pain transmission evoked by N/OFQ. The calculated full-length pre-protein of NIPSNAP1 was 33 kDa, whereas the N-terminal truncated form of NIPSNAP1 (29 kDa) was ubiquitously expressed in the neuronal tissues, especially in synaptic membrane and mitThe Journal of biological chemistry10403-10413https://www.ebi.ac.uk/biostudies/studies/S-EPMC3322983biostudies-literatureIdentification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission.PMC3322983Tsubouchi SNoda TMinami TIwamatsu AHanda HOkuda-Ashitaka EIto SKiyonari HfalseIdentification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission.4-Nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) is a molecule of physiologically unknown function, although it is predominantly expressed in the brain, spinal cord, liver, and kidney. We identified NIPSNAP1 as a protein that interacts with the neuropeptide nocistatin (NST) from synaptosomal membranes of mouse spinal cord using high-performance affinity latex beads. NST, which is produced from the same precursor protein as an opioid-like neuropeptide nociceptin/orphanin FQ (N/OFQ), has opposite effects on pain transmission evoked by N/OFQ. The calculated full-length pre-protein of NIPSNAP1 was 33 kDa, whereas the N-terminal truncated form of NIPSNAP1 (29 kDa) was ubiquitously expressed in the neuronal tissues, especially in synaptic membrane and mit2012-01-01T00:00:00Z2012 Mar2025-04-22T19:17:11.16Z2019-06-05T17:13:08ZS-EPMC33229832231198510.1074/jbc.M111.27186610.1074/jbc.m111.271866