{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Finnegan ML"],"funding":["NIGMS NIH HHS"],"pagination":["1969-78"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3328712"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["102(8)"],"pubmed_abstract":["The intrinsic polymer properties of glycine-rich sequences are evaluated with a set of iso-1-cytochrome c variants with N-terminal inserts of the sequence (GGGGGK)(n) for n = 1-5. The thermodynamics and kinetics of His-heme loop formation are measured as a function of guanidine hydrochloride (GdnHCl) concentration for loop sizes ranging from 22 to 46 residues. The scaling exponent for loop formation, ν(3), evaluated using the Jacobson-Stockmayer equation is near 1.8, at 1.5 and 3.0 M GdnHCl, but it increases to 2.2 in 6.0 M GdnHCl. Previous work on a set of iso-1-cytochrome c variants with (AAAAAK)(n) inserts gave ν(3) = 2.2 for alanine-rich sequences in both 3.0 and 6.0 M GdnHCl. Chain stiffness was evaluated from the relative magnitude of Flory's characteristic ratio, C(n), for alanine-r"],"journal":["Biophysical journal"],"pubmed_title":["Scaling properties of glycine-rich sequences in guanidine hydrochloride solutions."],"pmcid":["PMC3328712"],"funding_grant_id":["P20 GM103546","P20GM103546","R01 GM074750","GM074750-04S1"],"pubmed_authors":["Finnegan ML","Bowler BE"],"additional_accession":[]},"is_claimable":false,"name":"Scaling properties of glycine-rich sequences in guanidine hydrochloride solutions.","description":"The intrinsic polymer properties of glycine-rich sequences are evaluated with a set of iso-1-cytochrome c variants with N-terminal inserts of the sequence (GGGGGK)(n) for n = 1-5. The thermodynamics and kinetics of His-heme loop formation are measured as a function of guanidine hydrochloride (GdnHCl) concentration for loop sizes ranging from 22 to 46 residues. The scaling exponent for loop formation, ν(3), evaluated using the Jacobson-Stockmayer equation is near 1.8, at 1.5 and 3.0 M GdnHCl, but it increases to 2.2 in 6.0 M GdnHCl. Previous work on a set of iso-1-cytochrome c variants with (AAAAAK)(n) inserts gave ν(3) = 2.2 for alanine-rich sequences in both 3.0 and 6.0 M GdnHCl. Chain stiffness was evaluated from the relative magnitude of Flory's characteristic ratio, C(n), for alanine-r","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Apr","modification":"2025-05-29T21:27:12.368Z","creation":"2025-05-29T21:27:12.368Z"},"accession":"S-EPMC3328712","cross_references":{"pubmed":["22768954"],"doi":["10.1016/j.bpj.2012.03.049"]}}