{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu G"],"funding":["NCI NIH HHS"],"pagination":["5786-91"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC33291"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["98(10)"],"pubmed_abstract":["Proteinase inhibitor I (Inh I) and proteinase inhibitor II (Inh II) from potato tubers are effective proteinase inhibitors of chymotrypsin and trypsin. Inh I and Inh II were shown to suppress irradiation-induced transformation in mouse embryo fibroblasts suggesting that they possess anticarcinogenic characteristics. We have previously demonstrated that Inh I and Inh II could effectively block UV irradiation-induced activation of transcription activator protein 1 (AP-1) in mouse JB6 epidermal cells, which mechanistically may explain their anticarcinogenic actions. In the present study, we investigated the effects of Inh I and Inh II on the expression and composition pattern of the AP-1 complex following stimulation by UV B (UVB) irradiation in the JB6 model. We found that Inh I and Inh II specifically inhibited UVB-induced AP-1, but not NFkappaB, activity in JB6 cells. Both Inh I and Inh II up-regulated AP-1 constituent proteins, JunD and Fra-2, and suppressed c-Jun and c-Fos expression and composition in bound AP-1 in response to UVB stimulation. This regulation of the AP-1 protein compositional pattern in response to Inh I or Inh II may be critical for the inhibition of UVB-induced AP-1 activity by these agents found in potatoes."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Proteinase inhibitors I and II from potatoes block UVB-induced AP-1 activity by regulating the AP-1 protein compositional patterns in JB6 cells."],"pmcid":["PMC33291"],"funding_grant_id":["R01 CA077646","CA77646","R37 CA081064","R01 CA081064","CA74916","CA81064"],"pubmed_authors":["Chen N","Kaji A","Dong Z","Ryan CA","Bode AM","Liu G"],"additional_accession":[]},"is_claimable":false,"name":"Proteinase inhibitors I and II from potatoes block UVB-induced AP-1 activity by regulating the AP-1 protein compositional patterns in JB6 cells.","description":"Proteinase inhibitor I (Inh I) and proteinase inhibitor II (Inh II) from potato tubers are effective proteinase inhibitors of chymotrypsin and trypsin. Inh I and Inh II were shown to suppress irradiation-induced transformation in mouse embryo fibroblasts suggesting that they possess anticarcinogenic characteristics. We have previously demonstrated that Inh I and Inh II could effectively block UV irradiation-induced activation of transcription activator protein 1 (AP-1) in mouse JB6 epidermal cells, which mechanistically may explain their anticarcinogenic actions. In the present study, we investigated the effects of Inh I and Inh II on the expression and composition pattern of the AP-1 complex following stimulation by UV B (UVB) irradiation in the JB6 model. We found that Inh I and Inh II specifically inhibited UVB-induced AP-1, but not NFkappaB, activity in JB6 cells. Both Inh I and Inh II up-regulated AP-1 constituent proteins, JunD and Fra-2, and suppressed c-Jun and c-Fos expression and composition in bound AP-1 in response to UVB stimulation. This regulation of the AP-1 protein compositional pattern in response to Inh I or Inh II may be critical for the inhibition of UVB-induced AP-1 activity by these agents found in potatoes.","dates":{"release":"2001-01-01T00:00:00Z","publication":"2001 May","modification":"2025-05-29T19:21:54.38Z","creation":"2025-05-29T19:21:54.38Z"},"accession":"S-EPMC33291","cross_references":{"pubmed":["11331771"],"doi":["10.1073/pnas.101116298"]}}