biostudies-literatureHarris BSNCRR NIH HHSH1712-25https://www.ebi.ac.uk/biostudies/studies/S-EPMC3330807biostudies-literatureUnknown302(8)How chronic pressure overload affects the Purkinje fibers of the ventricular peripheral conduction system (PCS) is not known. Here, we used a connexin (Cx)40 knockout/enhanced green fluorescent protein knockin transgenic mouse model to specifically label the PCS. We hypothesized that the subendocardially located PCS would remodel after chronic pressure overload and therefore analyzed cell size, markers of hypertrophy, and PCS-specific Cx and ion channel expression patterns. Left ventricular hypertrophy with preserved systolic function was induced by 30 days of surgical transaortic constriction. After transaortic constriction, we observed that PCS cardiomyocytes hypertrophied by 23% (P < 0.05) and that microdissected PCS tissue exhibited upregulated markers of hypertrophy. PCS cardiomyocyteAmerican journal of physiology. Heart and circulatory physiologyRemodeling of the peripheral cardiac conduction system in response to pressure overload.PMC33308075-P20-RR-016434-010Gros DHarris BSO'Brien TXHaghshenas NBaicu CFScholz DRackley MSMukherjee RKasiganesan HMiquerol LfalseRemodeling of the peripheral cardiac conduction system in response to pressure overload.How chronic pressure overload affects the Purkinje fibers of the ventricular peripheral conduction system (PCS) is not known. Here, we used a connexin (Cx)40 knockout/enhanced green fluorescent protein knockin transgenic mouse model to specifically label the PCS. We hypothesized that the subendocardially located PCS would remodel after chronic pressure overload and therefore analyzed cell size, markers of hypertrophy, and PCS-specific Cx and ion channel expression patterns. Left ventricular hypertrophy with preserved systolic function was induced by 30 days of surgical transaortic constriction. After transaortic constriction, we observed that PCS cardiomyocytes hypertrophied by 23% (P < 0.05) and that microdissected PCS tissue exhibited upregulated markers of hypertrophy. PCS cardiomyocyte2012-01-01T00:00:00Z2012 Apr2026-05-03T04:33:11.654Z2026-04-07T18:54:47.715ZS-EPMC33308072230766510.1152/ajpheart.00621.2011