biostudies-literatureScott RANCATS NIH HHSBritish Heart FoundationNCRR NIH HHSNIDDK NIH HHSMedical Research CouncilNHLBI NIH HHSNHGRI NIH HHSNational Institute for Health Research (NIHR)Novo Nordisk Fonden1291-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC3331745biostudies-literatureUnknown61(5)Gene-lifestyle interactions have been suggested to contribute to the development of type 2 diabetes. Glucose levels 2 h after a standard 75-g glucose challenge are used to diagnose diabetes and are associated with both genetic and lifestyle factors. However, whether these factors interact to determine 2-h glucose levels is unknown. We meta-analyzed single nucleotide polymorphism (SNP) × BMI and SNP × physical activity (PA) interaction regression models for five SNPs previously associated with 2-h glucose levels from up to 22 studies comprising 54,884 individuals without diabetes. PA levels were dichotomized, with individuals below the first quintile classified as inactive (20%) and the remainder as active (80%). BMI was considered a continuous trait. Inactive individuals had higher 2-h gluDiabetesNo interactions between previously associated 2-hour glucose gene variants and physical activity or BMI on 2-hour glucose levels.PMC3331745MC_U147585824U1475000001U1475000002G0600717RG/07/008/23674P30 DK020572T32 HL007575UL1 RR025741R01 DK072041T32 HG000040G0902037G0100222MC_U106179473MC_UP_A620_1014MC_UP_A620_1015UL1 TR000124NNF11OC1014855MC_UP_A100_1003G1002084MC_U106179471G8802774NF-SI-0508-10082G0701863UL1 RR024148G19/35G0600717BK24 DK080140P30 DK063491Psaty BMFlorez JCChen YDSiscovick DSRice KDowling NFStancakova ATonjes AKao WHBornstein SRForouhi NGGroop LWalker MLoos RJHivert MFPankow JSScott RALaakso MBergmann SCollins FSFranks PWBouatia-Naji NJackson AUCarlson ODSandholt CHFox CSMeigs JBWatanabe RMTanaka TRasmussen-Torvik LJWareham NJYesupriya ABrage SBonnycastle LLWitte DRTeslovich TMKuusisto JLevy-Marchal CBoehnke MArking DEVollenweider PGrarup NHingorani ARenstrom FShrader PJohansson IBrunner ESchwarz PEManning AKDennison EMLyssenko VRotter JIDupuis JBoerwinkle EChu AYWilliams GHStumvoll MJonsson AProkopenko IWood ARFerrucci LShungin DGustafsson SHallmans GMarques-Vidal PLecoeur CLi GXie WBarroso IHu FBErdos MRChen HRybin DWaeber GLagou VMarek DCouper DJGoodarzi MOMuller GBarnes DEgan JSChines PSKutalik ZBumpstead SJMorken MAIngelsson EEkelund UKivimaki MIsomaa BGrassler JStringham HMKovacs PPedersen OLangenberg CPayne FHansen TMarmot MSayer AAJameson KAJorgensen TGlazer NLAadahl MCooper CFroguel PHolloway JWKumari MNorth KEfalseNo interactions between previously associated 2-hour glucose gene variants and physical activity or BMI on 2-hour glucose levels.Gene-lifestyle interactions have been suggested to contribute to the development of type 2 diabetes. Glucose levels 2 h after a standard 75-g glucose challenge are used to diagnose diabetes and are associated with both genetic and lifestyle factors. However, whether these factors interact to determine 2-h glucose levels is unknown. We meta-analyzed single nucleotide polymorphism (SNP) × BMI and SNP × physical activity (PA) interaction regression models for five SNPs previously associated with 2-h glucose levels from up to 22 studies comprising 54,884 individuals without diabetes. PA levels were dichotomized, with individuals below the first quintile classified as inactive (20%) and the remainder as active (80%). BMI was considered a continuous trait. Inactive individuals had higher 2-h glu2012-01-01T00:00:00Z2012 May2026-04-29T23:13:14.292Z2026-04-21T03:09:02.291ZS-EPMC33317452241587710.2337/db11-0973