{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lenco J"],"funding":["NCI NIH HHS"],"pagination":["7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3331852"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Naturally occurring native peptides provide important information about physiological states of an organism and its changes in disease conditions but protocols and methods for assessing their abundance are not well-developed. In this paper, we describe a simple procedure for the quantification of non-tryptic peptides in body fluids. The workflow includes an enrichment step followed by two-dimensional fractionation of native peptides and MS/MS data management facilitating the design and validation of LC- MRM MS assays. The added value of the workflow is demonstrated in the development of a triplex LC-MRM MS assay used for quantification of peptides potentially associated with the progression of liver disease to hepatocellular carcinoma."],"journal":["Proteome science"],"pubmed_title":["MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration."],"pmcid":["PMC3331852"],"funding_grant_id":["R01 CA135069"],"pubmed_authors":["Lenco J","Lan R","Goldman R","Edwards N"],"additional_accession":[]},"is_claimable":false,"name":"MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration.","description":"Naturally occurring native peptides provide important information about physiological states of an organism and its changes in disease conditions but protocols and methods for assessing their abundance are not well-developed. In this paper, we describe a simple procedure for the quantification of non-tryptic peptides in body fluids. The workflow includes an enrichment step followed by two-dimensional fractionation of native peptides and MS/MS data management facilitating the design and validation of LC- MRM MS assays. The added value of the workflow is demonstrated in the development of a triplex LC-MRM MS assay used for quantification of peptides potentially associated with the progression of liver disease to hepatocellular carcinoma.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Feb","modification":"2025-04-04T09:12:35.771Z","creation":"2019-03-27T00:52:31Z"},"accession":"S-EPMC3331852","cross_references":{"pubmed":["22304756"],"doi":["10.1186/1477-5956-10-7"]}}