biostudies-literatureUnknown7(5)Qin YSequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.PloS onee35819https://www.ebi.ac.uk/biostudies/studies/S-EPMC3344849biostudies-literatureSequencing by hybridization of long targets.PMC3344849Brenner MPSchneider TMQin YfalseSequencing by hybridization of long targets.Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.2012-01-01T00:00:00Z20122026-04-30T18:59:43.293Z2019-03-26T23:18:37ZS-EPMC33448492257412410.1371/journal.pone.0035819