biostudies-literatureKrishnamoorthy SNIGMS NIH HHS2018-27https://www.ebi.ac.uk/biostudies/studies/S-EPMC3349829biostudies-literatureUnknown180(5)Resolution of acute inflammation is an active process that involves the biosynthesis of specialized proresolving lipid mediators. Among them, resolvin D1 (RvD1) actions are mediated by two G protein-coupled receptors (GPCRs), ALX/FPR2 and GPR32, that also regulate specific microRNAs (miRNAs) and their target genes in novel resolution circuits. We report the ligand selectivity of RvD1 activation of ALX/FPR2 and GPR32. In addition to RvD1, its aspirin-triggered epimer and RvD1 analogs each dose dependently and effectively activated ALX/FPR2 and GPR32 in GPCR-overexpressing β-arrestin systems using luminescence and electric cell-substrate impedance sensing. To corroborate these findings in vivo, neutrophil infiltration in self-limited peritonitis was reduced in human ALX/FPR2-overexpressing tThe American journal of pathologyResolvin D1 receptor stereoselectivity and regulation of inflammation and proresolving microRNAs.PMC3349829R01-GM038765R01 GM038765Fredman GRecchiuti AKrishnamoorthy SSerhan CNChiang NfalseResolvin D1 receptor stereoselectivity and regulation of inflammation and proresolving microRNAs.Resolution of acute inflammation is an active process that involves the biosynthesis of specialized proresolving lipid mediators. Among them, resolvin D1 (RvD1) actions are mediated by two G protein-coupled receptors (GPCRs), ALX/FPR2 and GPR32, that also regulate specific microRNAs (miRNAs) and their target genes in novel resolution circuits. We report the ligand selectivity of RvD1 activation of ALX/FPR2 and GPR32. In addition to RvD1, its aspirin-triggered epimer and RvD1 analogs each dose dependently and effectively activated ALX/FPR2 and GPR32 in GPCR-overexpressing β-arrestin systems using luminescence and electric cell-substrate impedance sensing. To corroborate these findings in vivo, neutrophil infiltration in self-limited peritonitis was reduced in human ALX/FPR2-overexpressing t2012-01-01T00:00:00Z2012 May2025-04-05T15:41:18.467Z2019-03-27T00:53:22ZS-EPMC33498292244994810.1016/j.ajpath.2012.01.028