{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Acerbo AS"],"funding":["NIBIB NIH HHS","NCRR NIH HHS","NIAMS NIH HHS"],"pagination":["3607-13"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3364542"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84(8)"],"pubmed_abstract":["Fourier transform infrared microspectroscopy (FTIRM) is a widely used method for mapping the material properties of bone and other mineralized tissues, including mineralization, crystallinity, carbonate substitution, and collagen cross-linking. This technique is traditionally performed in a transmission-based geometry, which requires the preparation of plastic-embedded thin sections, limiting its functionality. Here, we theoretically and empirically demonstrate the development of reflection-based FTIRM as an alternative to the widely adopted transmission-based FTIRM, which reduces specimen preparation time and broadens the range of specimens that can be imaged. In this study, mature mouse femurs were plastic-embedded and longitudinal sections were cut at a thickness of 4 μm for transmissio"],"journal":["Analytical chemistry"],"pubmed_title":["Imaging the material properties of bone specimens using reflection-based infrared microspectroscopy."],"pmcid":["PMC3364542"],"funding_grant_id":["R01 AR052778","P30 EB009998","S10 RR023782-01","AR052778","S10 RR023782"],"pubmed_authors":["Carr GL","Miller LM","Judex S","Acerbo AS"],"additional_accession":[]},"is_claimable":false,"name":"Imaging the material properties of bone specimens using reflection-based infrared microspectroscopy.","description":"Fourier transform infrared microspectroscopy (FTIRM) is a widely used method for mapping the material properties of bone and other mineralized tissues, including mineralization, crystallinity, carbonate substitution, and collagen cross-linking. This technique is traditionally performed in a transmission-based geometry, which requires the preparation of plastic-embedded thin sections, limiting its functionality. Here, we theoretically and empirically demonstrate the development of reflection-based FTIRM as an alternative to the widely adopted transmission-based FTIRM, which reduces specimen preparation time and broadens the range of specimens that can be imaged. In this study, mature mouse femurs were plastic-embedded and longitudinal sections were cut at a thickness of 4 μm for transmissio","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Apr","modification":"2025-04-21T13:50:44.928Z","creation":"2019-03-27T00:54:00Z"},"accession":"S-EPMC3364542","cross_references":{"pubmed":["22455306"],"doi":["10.1021/ac203375d"]}}