{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li Y"],"funding":["NIAID NIH HHS"],"pagination":["8716-21"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3365160"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["109(22)"],"pubmed_abstract":["Maintenance of membrane function is essential and regulated at the genomic, transcriptional, and translational levels. Bacterial pathogens have a variety of mechanisms to adapt their membrane in response to transmission between environment, vector, and human host. Using a well-characterized model of lipid A diversification (Francisella), we demonstrate temperature-regulated membrane remodeling directed by multiple alleles of the lipid A-modifying N-acyltransferase enzyme, LpxD. Structural analysis of the lipid A at environmental and host temperatures revealed that the LpxD1 enzyme added a 3-OH C18 acyl group at 37 °C (host), whereas the LpxD2 enzyme added a 3-OH C16 acyl group at 18 °C (environment). Mutational analysis of either of the individual Francisella lpxD genes altered outer membr"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["LPS remodeling is an evolved survival strategy for bacteria."],"pmcid":["PMC3365160"],"funding_grant_id":["T32 AI007540","U54 AI057141"],"pubmed_authors":["Pelletier MR","Goodlett DR","Ernst RK","Li Y","Raetz CR","Rasko DA","Leszyk JD","Scott AJ","Masoudi A","Powell DA","Shaffer SA","Wang X"],"additional_accession":[]},"is_claimable":false,"name":"LPS remodeling is an evolved survival strategy for bacteria.","description":"Maintenance of membrane function is essential and regulated at the genomic, transcriptional, and translational levels. Bacterial pathogens have a variety of mechanisms to adapt their membrane in response to transmission between environment, vector, and human host. Using a well-characterized model of lipid A diversification (Francisella), we demonstrate temperature-regulated membrane remodeling directed by multiple alleles of the lipid A-modifying N-acyltransferase enzyme, LpxD. Structural analysis of the lipid A at environmental and host temperatures revealed that the LpxD1 enzyme added a 3-OH C18 acyl group at 37 °C (host), whereas the LpxD2 enzyme added a 3-OH C16 acyl group at 18 °C (environment). Mutational analysis of either of the individual Francisella lpxD genes altered outer membr","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 May","modification":"2025-04-04T23:31:07.766Z","creation":"2019-03-27T00:54:01Z"},"accession":"S-EPMC3365160","cross_references":{"pubmed":["22586119"],"doi":["10.1073/pnas.1202908109"]}}