biostudies-literatureBaker NNIA NIH HHSNIAMS NIH HHSBiotechnology and Biological Sciences Research Council2289-99https://www.ebi.ac.uk/biostudies/studies/S-EPMC3366172biostudies-literatureUnknown64(7)<h4>Objective</h4>Wnt-1-inducible signaling pathway protein 3 (WISP-3)/CCN6 is mutated in progressive pseudorheumatoid dysplasia and may have effects on cartilage homeostasis. The aim of this study was to ascertain additional roles for WISP-3/CCN6 by determining its expression in osteoarthritic (OA) cartilage and by investigating its effects on cartilage-relevant metalloproteinase expression in immortalized (C-28/I2) and primary chondrocytes.<h4>Methods</h4>Cartilage steady-state levels of WISP-3/CCN6 messenger RNA and protein production were determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. WISP-3/CCN6 was overexpressed in C-28/I2 cells, and the resultant clones were analyzed by quantitative RT-PCR. The stArthritis and rheumatismDual regulation of metalloproteinase expression in chondrocytes by Wnt-1-inducible signaling pathway protein 3/CCN6.PMC3366172RC4 AR060546-01RC4 AR060546R01 AG022021-09RC4-AR-060546R01-AG-022021R01 AG022021Sharpe PClements KMGavrilovic JBaker NCulley KBevan DOtero MBarker WNewham PLangham CJGoldring MBfalseDual regulation of metalloproteinase expression in chondrocytes by Wnt-1-inducible signaling pathway protein 3/CCN6.<h4>Objective</h4>Wnt-1-inducible signaling pathway protein 3 (WISP-3)/CCN6 is mutated in progressive pseudorheumatoid dysplasia and may have effects on cartilage homeostasis. The aim of this study was to ascertain additional roles for WISP-3/CCN6 by determining its expression in osteoarthritic (OA) cartilage and by investigating its effects on cartilage-relevant metalloproteinase expression in immortalized (C-28/I2) and primary chondrocytes.<h4>Methods</h4>Cartilage steady-state levels of WISP-3/CCN6 messenger RNA and protein production were determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. WISP-3/CCN6 was overexpressed in C-28/I2 cells, and the resultant clones were analyzed by quantitative RT-PCR. The st2012-01-01T00:00:00Z2012 Jul2025-04-03T22:33:42.535Z2019-03-27T00:54:06ZS-EPMC33661722229441510.1002/art.34411