{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Schroeder MA"],"funding":["British Heart Foundation","NIBIB NIH HHS","Medical Research Council","Wellcome Trust"],"pagination":["201-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3378498"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(2)"],"pubmed_abstract":["Background
Carnitine acetyltransferase catalyzes the reversible conversion of acetyl-coenzyme A (CoA) into acetylcarnitine. The aim of this study was to use the metabolic tracer hyperpolarized [2-(13)C]pyruvate with magnetic resonance spectroscopy to determine whether carnitine acetyltransferase facilitates carbohydrate oxidation in the heart.Methods and results
Ex vivo, following hyperpolarized [2-(13)C]pyruvate infusion, the [1-(13)C]acetylcarnitine resonance was saturated with a radiofrequency pulse, and the effect of this saturation on [1-(13)C]citrate and [5-(13)C]glutamate was observed. In vivo, [2-(13)C]pyruvate was infused into 3 groups of fed male Wistar rats: (1) controls, (2) rats in which dichloroacetate enhanced pyruvate dehydrogenase flux, and (3) rats in whic"],"journal":["Circulation. Cardiovascular imaging"],"pubmed_title":["The cycling of acetyl-coenzyme A through acetylcarnitine buffers cardiac substrate supply: a hyperpolarized 13C magnetic resonance study."],"pmcid":["PMC3378498"],"funding_grant_id":["F31 EB006692","PS/02/002/14893","G0601490","RG/07/004/22659","FS/10/002/28078","089010","PG/07/070/23365","1-F31-EB006692-01A1"],"pubmed_authors":["Clarke K","Tyler DJ","Atherton HJ","Lee P","Schroeder MA","Dodd MS","Radda GK","Cochlin LE"],"additional_accession":[]},"is_claimable":false,"name":"The cycling of acetyl-coenzyme A through acetylcarnitine buffers cardiac substrate supply: a hyperpolarized 13C magnetic resonance study.","description":"Background
Carnitine acetyltransferase catalyzes the reversible conversion of acetyl-coenzyme A (CoA) into acetylcarnitine. The aim of this study was to use the metabolic tracer hyperpolarized [2-(13)C]pyruvate with magnetic resonance spectroscopy to determine whether carnitine acetyltransferase facilitates carbohydrate oxidation in the heart.Methods and results
Ex vivo, following hyperpolarized [2-(13)C]pyruvate infusion, the [1-(13)C]acetylcarnitine resonance was saturated with a radiofrequency pulse, and the effect of this saturation on [1-(13)C]citrate and [5-(13)C]glutamate was observed. In vivo, [2-(13)C]pyruvate was infused into 3 groups of fed male Wistar rats: (1) controls, (2) rats in which dichloroacetate enhanced pyruvate dehydrogenase flux, and (3) rats in whic","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Mar","modification":"2025-04-04T18:56:08.5Z","creation":"2019-03-26T23:49:24Z"},"accession":"S-EPMC3378498","cross_references":{"pubmed":["22238215"],"doi":["10.1161/circimaging.111.969451","10.1161/CIRCIMAGING.111.969451"]}}