biostudies-literatureSchroeder MABritish Heart FoundationNIBIB NIH HHSMedical Research CouncilWellcome Trust201-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC3378498biostudies-literatureUnknown5(2)<h4>Background</h4>Carnitine acetyltransferase catalyzes the reversible conversion of acetyl-coenzyme A (CoA) into acetylcarnitine. The aim of this study was to use the metabolic tracer hyperpolarized [2-(13)C]pyruvate with magnetic resonance spectroscopy to determine whether carnitine acetyltransferase facilitates carbohydrate oxidation in the heart.<h4>Methods and results</h4>Ex vivo, following hyperpolarized [2-(13)C]pyruvate infusion, the [1-(13)C]acetylcarnitine resonance was saturated with a radiofrequency pulse, and the effect of this saturation on [1-(13)C]citrate and [5-(13)C]glutamate was observed. In vivo, [2-(13)C]pyruvate was infused into 3 groups of fed male Wistar rats: (1) controls, (2) rats in which dichloroacetate enhanced pyruvate dehydrogenase flux, and (3) rats in whicCirculation. Cardiovascular imagingThe cycling of acetyl-coenzyme A through acetylcarnitine buffers cardiac substrate supply: a hyperpolarized 13C magnetic resonance study.PMC3378498F31 EB006692PS/02/002/14893G0601490RG/07/004/22659FS/10/002/28078089010PG/07/070/233651-F31-EB006692-01A1Clarke KTyler DJAtherton HJLee PSchroeder MADodd MSRadda GKCochlin LEfalseThe cycling of acetyl-coenzyme A through acetylcarnitine buffers cardiac substrate supply: a hyperpolarized 13C magnetic resonance study.<h4>Background</h4>Carnitine acetyltransferase catalyzes the reversible conversion of acetyl-coenzyme A (CoA) into acetylcarnitine. The aim of this study was to use the metabolic tracer hyperpolarized [2-(13)C]pyruvate with magnetic resonance spectroscopy to determine whether carnitine acetyltransferase facilitates carbohydrate oxidation in the heart.<h4>Methods and results</h4>Ex vivo, following hyperpolarized [2-(13)C]pyruvate infusion, the [1-(13)C]acetylcarnitine resonance was saturated with a radiofrequency pulse, and the effect of this saturation on [1-(13)C]citrate and [5-(13)C]glutamate was observed. In vivo, [2-(13)C]pyruvate was infused into 3 groups of fed male Wistar rats: (1) controls, (2) rats in which dichloroacetate enhanced pyruvate dehydrogenase flux, and (3) rats in whic2012-01-01T00:00:00Z2012 Mar2025-04-04T18:56:08.5Z2019-03-26T23:49:24ZS-EPMC33784982223821510.1161/circimaging.111.96945110.1161/CIRCIMAGING.111.969451