biostudies-literatureUnknown21(14)Jaschke AThe uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine (Arfrp1(vil-/-)) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. Arfrp1(vil-/-) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted Human molecular genetics3128-42https://www.ebi.ac.uk/biostudies/studies/S-EPMC3384381biostudies-literatureThe GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.PMC3384381Heeren JMoser MBrigelius-Flohe RPetzke KJHesse DPuchkov DKoepsell HJoost HGZahn CChung BKluge RJaschke ASchurmann AfalseThe GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.The uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine (Arfrp1(vil-/-)) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. Arfrp1(vil-/-) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted 2012-01-01T00:00:00Z2012 Jul2025-04-05T13:05:24.093Z2019-03-27T00:55:01ZS-EPMC33843812250558510.1093/hmg/dds140