{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kulczyk AW"],"funding":["NIGMS NIH HHS"],"pagination":["9408-13"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3386087"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["109(24)"],"pubmed_abstract":["The lagging-strand DNA polymerase requires an oligoribonucleotide, synthesized by DNA primase, to initiate the synthesis of an Okazaki fragment. In the replication system of bacteriophage T7 both DNA primase and DNA helicase activities are contained within a single protein, the bifunctional gene 4 protein (gp4). Intermolecular interactions between gp4 and T7 DNA polymerase are crucial for the stabilization of the oligoribonucleotide, its transfer to the polymerase, and its extension by DNA polymerase. We have identified conditions necessary to assemble the T7 priming complex and characterized its biophysical properties using fluorescence anisotropy. In order to reveal molecular interactions that occur during delivery of the oligoribonucleotide to DNA polymerase, we have used four genetical"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Molecular interactions in the priming complex of bacteriophage T7."],"pmcid":["PMC3386087"],"funding_grant_id":["R01 GM054397","GM-54397"],"pubmed_authors":["Kulczyk AW","Richardson CC"],"additional_accession":[]},"is_claimable":false,"name":"Molecular interactions in the priming complex of bacteriophage T7.","description":"The lagging-strand DNA polymerase requires an oligoribonucleotide, synthesized by DNA primase, to initiate the synthesis of an Okazaki fragment. In the replication system of bacteriophage T7 both DNA primase and DNA helicase activities are contained within a single protein, the bifunctional gene 4 protein (gp4). Intermolecular interactions between gp4 and T7 DNA polymerase are crucial for the stabilization of the oligoribonucleotide, its transfer to the polymerase, and its extension by DNA polymerase. We have identified conditions necessary to assemble the T7 priming complex and characterized its biophysical properties using fluorescence anisotropy. In order to reveal molecular interactions that occur during delivery of the oligoribonucleotide to DNA polymerase, we have used four genetical","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jun","modification":"2025-04-05T13:19:35.971Z","creation":"2019-03-27T00:55:03Z"},"accession":"S-EPMC3386087","cross_references":{"pubmed":["22645372"],"doi":["10.1073/pnas.1207033109"]}}