{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Watrous J"],"funding":["NCRR NIH HHS","NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["E1743-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3387089"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["109(26)"],"pubmed_abstract":["Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a \"holy grail\" in microbiology. This work describes a highly sensitive, broadly applicable, and cost-effective approach that allows metabolic profiling of live microbial colonies directly from a Petri dish without any sample preparation. Nanospray desorption electrospray ionization mass spectrometry (MS), combined with alignment of MS data and molecular networking, enabled monitoring of metabolite production from live microbial colonies from diverse bacterial genera, including Bacillus subtilis, Streptomyces coelicolor, Mycobacterium smegmatis, and Pseudomonas aeruginosa. This work demonstrates that, by using these tools to visualize small molecular changes within bacterial interactions, ins"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Mass spectral molecular networking of living microbial colonies."],"pmcid":["PMC3387089"],"funding_grant_id":["GM085770","P41 RR024851","R01 AI095125","GM094802","1-P41-RR024851","AI095125","R01 GM085770","R01 GM094802"],"pubmed_authors":["Yang JY","Dorrestein PC","Watrous J","Bandeira N","Laskin J","Alexandrov T","van der Voort M","Roach P","Pogliano K","Gross H","Kersten RD","Heath BS","Raaijmakers JM","Moore BS"],"additional_accession":[]},"is_claimable":false,"name":"Mass spectral molecular networking of living microbial colonies.","description":"Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a \"holy grail\" in microbiology. This work describes a highly sensitive, broadly applicable, and cost-effective approach that allows metabolic profiling of live microbial colonies directly from a Petri dish without any sample preparation. Nanospray desorption electrospray ionization mass spectrometry (MS), combined with alignment of MS data and molecular networking, enabled monitoring of metabolite production from live microbial colonies from diverse bacterial genera, including Bacillus subtilis, Streptomyces coelicolor, Mycobacterium smegmatis, and Pseudomonas aeruginosa. This work demonstrates that, by using these tools to visualize small molecular changes within bacterial interactions, ins","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jun","modification":"2025-07-02T03:05:07.708Z","creation":"2025-07-02T03:05:07.708Z"},"accession":"S-EPMC3387089","cross_references":{"pubmed":["22586093"],"doi":["10.1073/pnas.1203689109"]}}