biostudies-literatureUnknown63(10)Clemente MRIn plants and other organisms, glutathione (GSH) biosynthesis is catalysed sequentially by γ-glutamylcysteine synthetase (γECS) and glutathione synthetase (GSHS). In legumes, homoglutathione (hGSH) can replace GSH and is synthesized by γECS and a specific homoglutathione synthetase (hGSHS). The subcellular localization of the enzymes was examined by electron microscopy in several legumes and gene expression was analysed in Lotus japonicus plants treated for 1-48 h with 50 μM of hormones. Immunogold localization studies revealed that γECS is confined to chloroplasts and plastids, whereas hGSHS is also in the cytosol. Addition of hormones caused differential expression of thiol synthetases in roots. After 24-48 h, abscisic and salicylic acids downregulated GSHS whereas jasmonic acid upregulaJournal of experimental botany3923-34https://www.ebi.ac.uk/biostudies/studies/S-EPMC3388825biostudies-literatureThiol synthetases of legumes: immunogold localization and differential gene regulation by phytohormones.PMC3388825Loscos JNavascues JJames EKGay MClemente MRBustos-Sanmamed PBecana MPerez-Rontome CfalseThiol synthetases of legumes: immunogold localization and differential gene regulation by phytohormones.In plants and other organisms, glutathione (GSH) biosynthesis is catalysed sequentially by γ-glutamylcysteine synthetase (γECS) and glutathione synthetase (GSHS). In legumes, homoglutathione (hGSH) can replace GSH and is synthesized by γECS and a specific homoglutathione synthetase (hGSHS). The subcellular localization of the enzymes was examined by electron microscopy in several legumes and gene expression was analysed in Lotus japonicus plants treated for 1-48 h with 50 μM of hormones. Immunogold localization studies revealed that γECS is confined to chloroplasts and plastids, whereas hGSHS is also in the cytosol. Addition of hormones caused differential expression of thiol synthetases in roots. After 24-48 h, abscisic and salicylic acids downregulated GSHS whereas jasmonic acid upregula2012-01-01T00:00:00Z2012 Jun2025-04-05T13:20:04.104Z2019-03-27T00:55:10ZS-EPMC33888252244242410.1093/jxb/ers083