{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["109(28)"],"submitter":["Li X"],"pubmed_abstract":["REPRESSOR OF SILENCING 1 (ROS1) is a DNA demethylation enzyme that was previously identified during a genetic screen for the silencing of both RD29A-LUC and 35S-NPTII transgenes on a T-DNA construct. Here we performed a genetic screen to identify additional mutants in which the 35S-NPTII transgene is silenced. We identified several alleles of ros1 and of the following components of the RNA-directed DNA methylation (RdDM) pathway: NRPD1 (the largest subunit of polymerase IV), RDR2, NRPE1 (the largest subunit of polymerase V), NRPD2, AGO4, and DMS3. Our results show that the silencing of 35S-NPTII in the RdDM pathway mutants is due to the reduced expression of ROS1 in the mutants. We also identified a putative histone acetyltransferase (ROS4) from the genetic screen. The acetyltransferase contains a PHD-finger domain that binds to unmethylated histone H3K4. The mutation in ROS4 led to reduction of H3K18 and H3K23 acetylation levels. We show that the silencing of 35S-NPTII and some transposable element genes was released by the ddm1 mutation but that this also required ROS4. Our study identifies a unique antisilencing factor, and reveals that the RdDM pathway has an antisilencing function due to its role in maintaining ROS1 expression."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["11425-30"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3396497"],"repository":["biostudies-literature"],"pubmed_title":["Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis."],"pmcid":["PMC3396497"],"pubmed_authors":["Li X","Qian W","Shen J","Wang C","Zhu JK","Gong Z","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis.","description":"REPRESSOR OF SILENCING 1 (ROS1) is a DNA demethylation enzyme that was previously identified during a genetic screen for the silencing of both RD29A-LUC and 35S-NPTII transgenes on a T-DNA construct. Here we performed a genetic screen to identify additional mutants in which the 35S-NPTII transgene is silenced. We identified several alleles of ros1 and of the following components of the RNA-directed DNA methylation (RdDM) pathway: NRPD1 (the largest subunit of polymerase IV), RDR2, NRPE1 (the largest subunit of polymerase V), NRPD2, AGO4, and DMS3. Our results show that the silencing of 35S-NPTII in the RdDM pathway mutants is due to the reduced expression of ROS1 in the mutants. We also identified a putative histone acetyltransferase (ROS4) from the genetic screen. The acetyltransferase contains a PHD-finger domain that binds to unmethylated histone H3K4. The mutation in ROS4 led to reduction of H3K18 and H3K23 acetylation levels. We show that the silencing of 35S-NPTII and some transposable element genes was released by the ddm1 mutation but that this also required ROS4. Our study identifies a unique antisilencing factor, and reveals that the RdDM pathway has an antisilencing function due to its role in maintaining ROS1 expression.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jul","modification":"2021-02-21T09:18:36Z","creation":"2019-03-27T00:55:35Z"},"accession":"S-EPMC3396497","cross_references":{"pubmed":["22733760"],"doi":["10.1073/pnas.1208557109"]}}