{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lesourne R"],"funding":["Intramural NIH HHS"],"pagination":["1154-61"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3401357"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["189(3)"],"pubmed_abstract":["Themis1, a recently identified T cell protein, has a critical function in the generation of mature CD4(+)CD8(-) and CD4(-)CD8(+) (CD4 and CD8 single-positive [SP]) thymocytes and T cells. Although Themis1 has been shown to bind to the adaptor proteins LAT and Grb2, previous studies have yielded conflicting results regarding whether thymocytes from Themis1(-/-) mice exhibit TCR-mediated signaling defects. In this study, we demonstrate that, in the absence of Themis1, TCR-mediated signaling is selectively impaired in CD4 SP and CD8 SP thymocytes but is not affected in CD4(+)CD8(+) double-positive thymocytes despite high expression of Themis1 in double-positive thymocytes. Like Themis1, Themis2, a related member of the Themis family, which is expressed in B cells and macrophages, contains two"],"journal":["Journal of immunology (Baltimore, Md. : 1950)"],"pubmed_title":["Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function."],"pmcid":["PMC3401357"],"funding_grant_id":["ZIA HD001803"],"pubmed_authors":["Barr VA","Lesourne R","El-Khoury D","Love PE","Uehara S","Song KD","Samelson LE","Zvezdova E"],"additional_accession":[]},"is_claimable":false,"name":"Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.","description":"Themis1, a recently identified T cell protein, has a critical function in the generation of mature CD4(+)CD8(-) and CD4(-)CD8(+) (CD4 and CD8 single-positive [SP]) thymocytes and T cells. Although Themis1 has been shown to bind to the adaptor proteins LAT and Grb2, previous studies have yielded conflicting results regarding whether thymocytes from Themis1(-/-) mice exhibit TCR-mediated signaling defects. In this study, we demonstrate that, in the absence of Themis1, TCR-mediated signaling is selectively impaired in CD4 SP and CD8 SP thymocytes but is not affected in CD4(+)CD8(+) double-positive thymocytes despite high expression of Themis1 in double-positive thymocytes. Like Themis1, Themis2, a related member of the Themis family, which is expressed in B cells and macrophages, contains two","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Aug","modification":"2026-04-07T16:38:38.218Z","creation":"2019-03-27T00:55:48Z"},"accession":"S-EPMC3401357","cross_references":{"pubmed":["22732588"],"doi":["10.4049/jimmunol.1200123"]}}