biostudies-literatureMeguro AIntramural NIH HHS1557-65https://www.ebi.ac.uk/biostudies/studies/S-EPMC3401481biostudies-literatureUnknown46(12)Previously, we have shown that IL-21R(-/-) splenocytes ameliorate GVHD as compared with wild-type splenocytes. Here, we investigated whether or not IL-21R(-/-) splenocytes diminish the graft-versus-leukemia (GVL) effect. Surprisingly, IL-21R(-/-) splenocytes efficiently eliminate leukemic cells as well as wild-type splenocytes, suggesting the retention of GVL effects in the absence of IL-21 signaling. To compare the GVL effect between IL-21R(-/-) and wild-type cells, we titrated the number of splenocytes required for the elimination of leukemic cells and found that the threshold of GVL effect was obtained between 5 × 10(5) and 5 × 10(6) with both types of splenocytes. Cotransplantation with CD8-depleted splenocytes but not with purified CD8 T-cells resulted in a significant reduction in anBone marrow transplantationLack of IL-21 signal attenuates graft-versus-leukemia effect in the absence of CD8 T-cells.PMC3401481ZIA HL005408-03Hatanaka KOzawa KTatara ROh ISakata YLeonard WJOhmori TNakae SMatsu HSudo KOzaki KSato KMeguro AfalseLack of IL-21 signal attenuates graft-versus-leukemia effect in the absence of CD8 T-cells.Previously, we have shown that IL-21R(-/-) splenocytes ameliorate GVHD as compared with wild-type splenocytes. Here, we investigated whether or not IL-21R(-/-) splenocytes diminish the graft-versus-leukemia (GVL) effect. Surprisingly, IL-21R(-/-) splenocytes efficiently eliminate leukemic cells as well as wild-type splenocytes, suggesting the retention of GVL effects in the absence of IL-21 signaling. To compare the GVL effect between IL-21R(-/-) and wild-type cells, we titrated the number of splenocytes required for the elimination of leukemic cells and found that the threshold of GVL effect was obtained between 5 × 10(5) and 5 × 10(6) with both types of splenocytes. Cotransplantation with CD8-depleted splenocytes but not with purified CD8 T-cells resulted in a significant reduction in an2011-01-01T00:00:00Z2011 Dec2025-04-04T21:17:33.083Z2019-03-27T00:55:49ZS-EPMC34014812125842310.1038/bmt.2010.342