biostudies-literatureMeriin ABNIGMS NIH HHS2665-74https://www.ebi.ac.uk/biostudies/studies/S-EPMC3403235biostudies-literatureUnknown125(Pt 11)Aggresome formation is initiated upon proteasome failure, and facilitates autophagic clearance of protein aggregates to protect cells from proteotoxicity. Here we demonstrate that proteasome inhibition generates a signaling event to trigger aggresome formation. In aggresome signaling, the cell senses a build-up of aberrant newly synthesized proteins. The translation elongation factor eEF1A associated with these species, and knockdown of this factor suppressed aggresome formation. We used the Legionella toxin SidI to distinguish between the function of eEF1A in translation and its novel function in the aggresome formation. In fact, although it strongly inhibited translation, this toxin had only a marginal effect on aggresome formation. Furthermore, SidI reduced the threshold of the aberrantJournal of cell scienceAssociation of translation factor eEF1A with defective ribosomal products generates a signal for aggresome formation.PMC3403235R01GM086890R01 GM086890Meriin ABSherman MYZaarur NfalseAssociation of translation factor eEF1A with defective ribosomal products generates a signal for aggresome formation.Aggresome formation is initiated upon proteasome failure, and facilitates autophagic clearance of protein aggregates to protect cells from proteotoxicity. Here we demonstrate that proteasome inhibition generates a signaling event to trigger aggresome formation. In aggresome signaling, the cell senses a build-up of aberrant newly synthesized proteins. The translation elongation factor eEF1A associated with these species, and knockdown of this factor suppressed aggresome formation. We used the Legionella toxin SidI to distinguish between the function of eEF1A in translation and its novel function in the aggresome formation. In fact, although it strongly inhibited translation, this toxin had only a marginal effect on aggresome formation. Furthermore, SidI reduced the threshold of the aberrant2012-01-01T00:00:00Z2012 Jun2025-04-05T13:00:40.955Z2019-03-27T00:55:52ZS-EPMC34032352235795210.1242/jcs.098954