{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sehrawat S"],"funding":["NICHD NIH HHS","NIAID NIH HHS"],"pagination":["461-71"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3406328"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1(5)"],"pubmed_abstract":["To study the CD8(+) T cell response against a mouse γ-herpes virus, we generated K(b)-MHV-68-ORF8(604-612)RAG(-/-) CD8(+) T cell receptor transnuclear (TN) mice as a source of virus-specific CD8(+) T cells. K(b)-ORF8-Tet(+) CD8(+) T cells, expanded in the course of a resolving MHV-68 infection, served as a source of nucleus donors. Various in vivo and ex vivo assay criteria demonstrated the fine specificity and functionality of TN cells. TN cells proliferated extensively in response to viral infection, helped control viral burden, and exhibited a phenotype similar to that of endogenous K(b)-ORF8-Tet(+) cells. When compared to OT-1 cells, TN cells displayed distinct properties in response to lymphopenia and cognate antigen stimulation, which may be attributable to the affinity of the TCR ex"],"journal":["Cell reports"],"pubmed_title":["CD8(+) T cells from mice transnuclear for a TCR that recognizes a single H-2K(b)-restricted MHV68 epitope derived from gB-ORF8 help control infection."],"pmcid":["PMC3406328"],"funding_grant_id":["R37 HD045022","R01 HD045022","R01 AI033456"],"pubmed_authors":["Marques S","Koenig PA","Simas JP","Sehrawat S","Kirak O","Ploegh HL","Isaacson MK","Bozkurt G","Jaenisch R"],"additional_accession":[]},"is_claimable":false,"name":"CD8(+) T cells from mice transnuclear for a TCR that recognizes a single H-2K(b)-restricted MHV68 epitope derived from gB-ORF8 help control infection.","description":"To study the CD8(+) T cell response against a mouse γ-herpes virus, we generated K(b)-MHV-68-ORF8(604-612)RAG(-/-) CD8(+) T cell receptor transnuclear (TN) mice as a source of virus-specific CD8(+) T cells. K(b)-ORF8-Tet(+) CD8(+) T cells, expanded in the course of a resolving MHV-68 infection, served as a source of nucleus donors. Various in vivo and ex vivo assay criteria demonstrated the fine specificity and functionality of TN cells. TN cells proliferated extensively in response to viral infection, helped control viral burden, and exhibited a phenotype similar to that of endogenous K(b)-ORF8-Tet(+) cells. When compared to OT-1 cells, TN cells displayed distinct properties in response to lymphopenia and cognate antigen stimulation, which may be attributable to the affinity of the TCR ex","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 May","modification":"2026-05-02T07:26:28.28Z","creation":"2026-04-07T17:45:53.937Z"},"accession":"S-EPMC3406328","cross_references":{"pubmed":["22832272"],"doi":["10.1016/j.celrep.2012.03.009"]}}