{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Byrum SD"],"funding":["NCATS NIH HHS","NCRR NIH HHS","NIDA NIH HHS","NIGMS NIH HHS","PHS HHS"],"pagination":["198-205"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3408609"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(1)"],"pubmed_abstract":["The field of epigenomics has been transformed by chromatin immunoprecipitation approaches that provide for the localization of a defined protein or posttranslationally modified protein to specific chromosomal sites. While these approaches have helped us conceptualize epigenetic mechanisms, the field has been limited by the inability to define features such as the proteome and histone modifications at a specific genomic locus in an unbiased manner. We developed an unbiased approach whereby a unique native genomic locus was isolated, which was followed by high-resolution proteomic identification of specifically associated proteins and histone posttranslational modifications. This chromatin affinity purification with mass spectrometry (ChAP-MS) technique was used to specifically enrich a ~1,0"],"journal":["Cell reports"],"pubmed_title":["ChAP-MS: a method for identification of proteins and histone posttranslational modifications at a single genomic locus."],"pmcid":["PMC3408609"],"funding_grant_id":["R01 DA025755","P20 RR015569","F32GM093614","UL1TR000039","P30 GM103450","UL1 TR000039","P20RR015569","U54 GM103520","U54RR020839","F32 GM093614","U54 RR020839","R01DA025755","P20 RR016460","P20RR016460"],"pubmed_authors":["Taverna SD","Byrum SD","Raman A","Tackett AJ"],"additional_accession":[]},"is_claimable":false,"name":"ChAP-MS: a method for identification of proteins and histone posttranslational modifications at a single genomic locus.","description":"The field of epigenomics has been transformed by chromatin immunoprecipitation approaches that provide for the localization of a defined protein or posttranslationally modified protein to specific chromosomal sites. While these approaches have helped us conceptualize epigenetic mechanisms, the field has been limited by the inability to define features such as the proteome and histone modifications at a specific genomic locus in an unbiased manner. We developed an unbiased approach whereby a unique native genomic locus was isolated, which was followed by high-resolution proteomic identification of specifically associated proteins and histone posttranslational modifications. This chromatin affinity purification with mass spectrometry (ChAP-MS) technique was used to specifically enrich a ~1,0","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jul","modification":"2025-04-05T10:47:34.108Z","creation":"2019-03-27T00:56:08Z"},"accession":"S-EPMC3408609","cross_references":{"pubmed":["22840409"],"doi":["10.1016/j.celrep.2012.06.019"]}}