biostudies-literatureOseroff CNIAID NIH HHS1800-11https://www.ebi.ac.uk/biostudies/studies/S-EPMC3411923biostudies-literatureUnknown189(4)A panel of 133 allergens derived from 28 different sources, including fungi, trees, grasses, weeds, and indoor allergens, was surveyed utilizing prediction of HLA class II-binding peptides and ELISPOT assays with PBMC from allergic donors, resulting in the identification of 257 T cell epitopes. More than 90% of the epitopes were novel, and for 14 allergen sources were the first ever identified to our knowledge. The epitopes identified in the different allergen sources summed up to a variable fraction of the total extract response. In cases of allergens in which the identified T cell epitopes accounted for a minor fraction of the extract response, fewer known protein sequences were available, suggesting that for low epitope coverage allergen sources, additional allergen proteins remain to bJournal of immunology (Baltimore, Md. : 1950)T cell responses to known allergen proteins are differently polarized and account for a variable fraction of total response to allergen extracts.PMC3411923U19AI100275N01 AI040023R37 AI038425R01 AI091614U19 AI100275McKinney DMBrodie TMSallusto FAlam RPeters BSette ASidney JVita ROseroff CKolla RSouthwood SGrey HBroide DTripple VGreenbaum JAfalseT cell responses to known allergen proteins are differently polarized and account for a variable fraction of total response to allergen extracts.A panel of 133 allergens derived from 28 different sources, including fungi, trees, grasses, weeds, and indoor allergens, was surveyed utilizing prediction of HLA class II-binding peptides and ELISPOT assays with PBMC from allergic donors, resulting in the identification of 257 T cell epitopes. More than 90% of the epitopes were novel, and for 14 allergen sources were the first ever identified to our knowledge. The epitopes identified in the different allergen sources summed up to a variable fraction of the total extract response. In cases of allergens in which the identified T cell epitopes accounted for a minor fraction of the extract response, fewer known protein sequences were available, suggesting that for low epitope coverage allergen sources, additional allergen proteins remain to b2012-01-01T00:00:00Z2012 Aug2025-04-22T07:45:38.587Z2019-03-26T23:42:57ZS-EPMC34119232278676810.4049/jimmunol.1200850