{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["78(14)"],"submitter":["Cude WN"],"pubmed_abstract":["Members of the Roseobacter lineage of marine bacteria are prolific surface colonizers in marine coastal environments, and antimicrobial secondary metabolite production has been hypothesized to provide a competitive advantage to colonizing roseobacters. Here, we report that the roseobacter Phaeobacter sp. strain Y4I produces the blue pigment indigoidine via a nonribosomal peptide synthase (NRPS)-based biosynthetic pathway encoded by a novel series of genetically linked genes: igiBCDFE. A Tn5-based random mutagenesis library of Y4I showed a perfect correlation between indigoidine production by the Phaeobacter strain and inhibition of Vibrio fischeri on agar plates, revealing a previously unrecognized bioactivity of this molecule. In addition, igiD null mutants (igiD encoding the indigoidine "],"journal":["Applied and environmental microbiology"],"pagination":["4771-80"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3416362"],"repository":["biostudies-literature"],"pubmed_title":["Production of the antimicrobial secondary metabolite indigoidine contributes to competitive surface colonization by the marine roseobacter Phaeobacter sp. strain Y4I."],"pmcid":["PMC3416362"],"pubmed_authors":["May AL","Frank AM","Mooney J","Hadden MK","Buchan A","Cude WN","Gulvik CA","Tavanaei AA"],"additional_accession":[]},"is_claimable":false,"name":"Production of the antimicrobial secondary metabolite indigoidine contributes to competitive surface colonization by the marine roseobacter Phaeobacter sp. strain Y4I.","description":"Members of the Roseobacter lineage of marine bacteria are prolific surface colonizers in marine coastal environments, and antimicrobial secondary metabolite production has been hypothesized to provide a competitive advantage to colonizing roseobacters. Here, we report that the roseobacter Phaeobacter sp. strain Y4I produces the blue pigment indigoidine via a nonribosomal peptide synthase (NRPS)-based biosynthetic pathway encoded by a novel series of genetically linked genes: igiBCDFE. A Tn5-based random mutagenesis library of Y4I showed a perfect correlation between indigoidine production by the Phaeobacter strain and inhibition of Vibrio fischeri on agar plates, revealing a previously unrecognized bioactivity of this molecule. In addition, igiD null mutants (igiD encoding the indigoidine ","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jul","modification":"2026-04-29T16:31:19.443Z","creation":"2019-03-27T00:56:38Z"},"accession":"S-EPMC3416362","cross_references":{"pubmed":["22582055"],"doi":["10.1128/aem.00297-12","10.1128/AEM.00297-12"]}}