{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lu D"],"funding":["Medical Research Council","Wellcome Trust"],"pagination":["e40938"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3422229"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(8)"],"pubmed_abstract":["<h4>Background</h4>miRNAs are a class of small non-coding RNAs that regulate gene expression and have critical functions in various biological processes. Hundreds of miRNAs have been identified in mammalian genomes but only a small number of them have been functionally characterized. Recent studies also demonstrate that some miRNAs have important roles in reprogramming somatic cells to induced pluripotent stem cells (iPSCs).<h4>Methods</h4>We screened 52 miRNAs cloned in a piggybac (PB) vector for their roles in reprogramming of mouse embryonic fibroblast cells to iPSCs. To identify targets of miRNAs, we made Dgcr8-deficient embryonic stem (ES) cells and introduced miRNA mimics to these cells, which lack miRNA biogenesis. The direct target genes of miRNA were identified through global gene"],"journal":["PloS one"],"pubmed_title":["MiR-25 regulates Wwp2 and Fbxw7 and promotes reprogramming of mouse fibroblast cells to iPSCs."],"pmcid":["PMC3422229"],"funding_grant_id":["G1001781","G0700287","077186/Z/05/Z"],"pubmed_authors":["Campos LS","Vigorito E","Skarnes WC","Davis MP","Abreu-Goodger C","Dunham I","Liu P","Lu D","Wang W","Siede J","Enright AJ"],"additional_accession":[]},"is_claimable":false,"name":"MiR-25 regulates Wwp2 and Fbxw7 and promotes reprogramming of mouse fibroblast cells to iPSCs.","description":"<h4>Background</h4>miRNAs are a class of small non-coding RNAs that regulate gene expression and have critical functions in various biological processes. Hundreds of miRNAs have been identified in mammalian genomes but only a small number of them have been functionally characterized. Recent studies also demonstrate that some miRNAs have important roles in reprogramming somatic cells to induced pluripotent stem cells (iPSCs).<h4>Methods</h4>We screened 52 miRNAs cloned in a piggybac (PB) vector for their roles in reprogramming of mouse embryonic fibroblast cells to iPSCs. To identify targets of miRNAs, we made Dgcr8-deficient embryonic stem (ES) cells and introduced miRNA mimics to these cells, which lack miRNA biogenesis. The direct target genes of miRNA were identified through global gene","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012","modification":"2026-05-04T00:38:44.039Z","creation":"2026-04-07T19:53:06.633Z"},"accession":"S-EPMC3422229","cross_references":{"pubmed":["22912667"],"doi":["10.1371/journal.pone.0040938"]}}