biostudies-literatureUnknown3(2)Rossi SPNA probes for the specific detection of DNA from olive oil samples by microarray technology were developed. The presence of as low as 5% refined hazelnut (Corylus avellana) oil in extra-virgin olive oil (Olea europaea L.) could be detected by using a PNA microarray. A set of two single nucleotide polymorphisms (SNPs) from the Actin gene of Olive was chosen as a model for evaluating the ability of PNA probes for discriminating olive cultivars. Both unmodified and C2-modified PNAs bearing an arginine side-chain were used, the latter showing higher sequence specificity. DNA extracted from leaves of three different cultivars (Ogliarola leccese, Canino and Frantoio) could be easily discriminated using a microarray with unmodified PNA probes, whereas discrimination of DNA from oil samples was more challenging, and could be obtained only by using chiral PNA probes.Artificial DNA, PNA & XNA63-72https://www.ebi.ac.uk/biostudies/studies/S-EPMC3429532biostudies-literatureSelective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays.PMC3429532Tedeschi TBaldoni LSforza SMarchelli RCorradini RCalabretta ARossi SArcioni SfalseSelective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays.PNA probes for the specific detection of DNA from olive oil samples by microarray technology were developed. The presence of as low as 5% refined hazelnut (Corylus avellana) oil in extra-virgin olive oil (Olea europaea L.) could be detected by using a PNA microarray. A set of two single nucleotide polymorphisms (SNPs) from the Actin gene of Olive was chosen as a model for evaluating the ability of PNA probes for discriminating olive cultivars. Both unmodified and C2-modified PNAs bearing an arginine side-chain were used, the latter showing higher sequence specificity. DNA extracted from leaves of three different cultivars (Ogliarola leccese, Canino and Frantoio) could be easily discriminated using a microarray with unmodified PNA probes, whereas discrimination of DNA from oil samples was more challenging, and could be obtained only by using chiral PNA probes.2012-01-01T00:00:00Z2012 Apr-Jun2024-11-12T11:56:17.045Z2019-03-27T00:57:15ZS-EPMC34295322277203810.4161/adna.20603