{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Koopmann R"],"funding":["NIGMS NIH HHS"],"pagination":["259-62"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3429599"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(3)"],"pubmed_abstract":["Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology."],"journal":["Nature methods"],"pubmed_title":["In vivo protein crystallization opens new routes in structural biology."],"pmcid":["PMC3429599"],"funding_grant_id":["R01 GM095583"],"pubmed_authors":["Martin AV","Andreasson J","Doak RB","Weidenspointner G","Caleman C","Barty A","Betzel C","Ullrich J","Williams GJ","Rolles D","Rudenko A","Schulz J","Shoeman RL","Liang M","Maia FR","Timneanu N","Wang X","Chapman HN","Stellato F","Schlichting I","Messerschmidt M","Kimmel N","Cupelli K","Hajdu J","Fromme P","White TA","Kassemeyer S","Bostedt C","Hampton CY","Stern S","Reich C","Bozek JD","Hauser G","Wunderer CB","Bogan MJ","Koopmann R","Rudek B","Epp SW","Graafsma H","Kirian RA","Weierstall U","Stehle T","Hirsemann H","Spence JC","Duszenko M","Soltau H","Deponte DP","Davidsson J","Aquila A","Hartmann R","Lomb L","Gumprecht L","Sierra RG","Erk B","Redecke L","Seibert MM","Fleckenstein H","Rehders D","Hartmann A","Hunter MS","Ekeberg T","Struder L","Barthelmess M","Bajt S","Boutet S","Coppola N","Nass K","Foucar L","Holl P"],"additional_accession":[]},"is_claimable":false,"name":"In vivo protein crystallization opens new routes in structural biology.","description":"Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jan","modification":"2026-04-29T13:25:12.27Z","creation":"2026-04-07T15:15:09.646Z"},"accession":"S-EPMC3429599","cross_references":{"pubmed":["22286384"],"doi":["10.1038/nmeth.1859"]}}