biostudies-literatureUnknown11(9)Michalik SThe cellular amount of proteins not only depends on synthesis but also on degradation. Here, we expand the understanding of differential protein levels by complementing synthesis data with a proteome-wide, mass spectrometry-based stable isotope labeling with amino acids in cell culture analysis of protein degradation in the human pathogen Staphylococcus aureus during glucose starvation. Monitoring protein stability profiles in a wild type and an isogenic clpP protease mutant revealed that 1) proteolysis mainly affected proteins with vegetative functions, anabolic and selected catabolic enzymes, whereas the expression of TCA cycle and gluconeogenesis enzymes increased; 2) most proteins were prone to aggregation in the clpP mutant; 3) the absence of ClpP correlated with protein denaturation Molecular & cellular proteomics : MCP558-70https://www.ebi.ac.uk/biostudies/studies/S-EPMC3434780biostudies-literatureLife and death of proteins: a case study of glucose-starved Staphylococcus aureus.PMC3434780Becher DKock HHecker MOtto AMoche MMeyer HLalk MSchurmann CSchluter RBernhardt JGerth UMichalik SfalseLife and death of proteins: a case study of glucose-starved Staphylococcus aureus.The cellular amount of proteins not only depends on synthesis but also on degradation. Here, we expand the understanding of differential protein levels by complementing synthesis data with a proteome-wide, mass spectrometry-based stable isotope labeling with amino acids in cell culture analysis of protein degradation in the human pathogen Staphylococcus aureus during glucose starvation. Monitoring protein stability profiles in a wild type and an isogenic clpP protease mutant revealed that 1) proteolysis mainly affected proteins with vegetative functions, anabolic and selected catabolic enzymes, whereas the expression of TCA cycle and gluconeogenesis enzymes increased; 2) most proteins were prone to aggregation in the clpP mutant; 3) the absence of ClpP correlated with protein denaturation 2012-01-01T00:00:00Z2012 Sep2025-04-18T18:25:49.389Z2019-03-27T00:57:32ZS-EPMC34347802255627910.1074/mcp.M112.01700410.1074/mcp.m112.017004