{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kurz M"],"funding":["Austrian Science Fund FWF","Austrian Science fund"],"pagination":["48-55"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3437442"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["168(1-2)"],"pubmed_abstract":["The replication of tick-borne encephalitis virus (TBEV), like that of all flaviviruses, is absolutely dependent on proteolytic processing. Production of the mature proteins C and prM from their common precursor requires the activity of the viral NS2B/3 protease (NS2B/3(pro)) at the C-terminus of protein C and the host signal peptidase I (SPaseI) at the N-terminus of protein prM. Recently, we have shown in cell culture that the cleavage of protein C and the subsequent production of TBEV particles can be made dependent on the activity of the foot-and-mouth disease virus 3C protease, but not on the activity of the HIV-1 protease (HIV1(pro)) (Schrauf et al., 2012). To investigate this failure, we developed an in vitro cleavage assay to assess the two cleavage reactions performed on the C-prM p"],"journal":["Virus research"],"pubmed_title":["NS2B/3 proteolysis at the C-prM junction of the tick-borne encephalitis virus polyprotein is highly membrane dependent."],"pmcid":["PMC3437442"],"funding_grant_id":["P 19528","FWF-P19528"],"pubmed_authors":["Zhu J","Stefan N","Skern T","Kurz M"],"additional_accession":[]},"is_claimable":false,"name":"NS2B/3 proteolysis at the C-prM junction of the tick-borne encephalitis virus polyprotein is highly membrane dependent.","description":"The replication of tick-borne encephalitis virus (TBEV), like that of all flaviviruses, is absolutely dependent on proteolytic processing. Production of the mature proteins C and prM from their common precursor requires the activity of the viral NS2B/3 protease (NS2B/3(pro)) at the C-terminus of protein C and the host signal peptidase I (SPaseI) at the N-terminus of protein prM. Recently, we have shown in cell culture that the cleavage of protein C and the subsequent production of TBEV particles can be made dependent on the activity of the foot-and-mouth disease virus 3C protease, but not on the activity of the HIV-1 protease (HIV1(pro)) (Schrauf et al., 2012). To investigate this failure, we developed an in vitro cleavage assay to assess the two cleavage reactions performed on the C-prM p","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Sep","modification":"2026-04-29T11:43:40.613Z","creation":"2019-03-27T00:57:43Z"},"accession":"S-EPMC3437442","cross_references":{"pubmed":["22727684"],"doi":["10.1016/j.virusres.2012.06.012"]}}