{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Xu DL"],"pubmed_abstract":["<h4>Background</h4>Aegilops variabilis No.1 is highly resistant to cereal cyst nematode (CCN). However, a lack of genomic information has restricted studies on CCN resistance genes in Ae. variabilis and has limited genetic applications in wheat breeding.<h4>Results</h4>Using RNA-Seq technology, we generated a root transcriptome at a sequencing depth of 4.69 gigabases of Ae. variabilis No. 1 from a pooled RNA sample. The sample contained equal amounts of RNA extracted from CCN-infected and untreated control plants at three time-points. Using the Trinity method, nearly 52,081,238 high-quality trimmed reads were assembled into a non-redundant set of 118,064 unigenes with an average length of 500 bp and an N50 of 599 bp. The total assembly was 59.09 Mb of unique transcriptome sequences with av"],"journal":["BMC genomics"],"pagination":["133"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3439707"],"repository":["biostudies-literature"],"pubmed_title":["De novo assembly and characterization of the root transcriptome of Aegilops variabilis during an interaction with the cereal cyst nematode."],"pmcid":["PMC3439707"],"pubmed_authors":["Pan ZF","Yu MQ","Long H","Zhang J","Xu DL","Liang JJ","Li JL","Chen X","Deng GB"],"additional_accession":[]},"is_claimable":false,"name":"De novo assembly and characterization of the root transcriptome of Aegilops variabilis during an interaction with the cereal cyst nematode.","description":"<h4>Background</h4>Aegilops variabilis No.1 is highly resistant to cereal cyst nematode (CCN). However, a lack of genomic information has restricted studies on CCN resistance genes in Ae. variabilis and has limited genetic applications in wheat breeding.<h4>Results</h4>Using RNA-Seq technology, we generated a root transcriptome at a sequencing depth of 4.69 gigabases of Ae. variabilis No. 1 from a pooled RNA sample. The sample contained equal amounts of RNA extracted from CCN-infected and untreated control plants at three time-points. Using the Trinity method, nearly 52,081,238 high-quality trimmed reads were assembled into a non-redundant set of 118,064 unigenes with an average length of 500 bp and an N50 of 599 bp. The total assembly was 59.09 Mb of unique transcriptome sequences with av","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Apr","modification":"2026-04-29T13:53:11.077Z","creation":"2026-04-07T15:15:32.285Z"},"accession":"S-EPMC3439707","cross_references":{"pubmed":["22494814"],"doi":["10.1186/1471-2164-13-133"]}}