biostudies-literatureUnknown13Xu DL<h4>Background</h4>Aegilops variabilis No.1 is highly resistant to cereal cyst nematode (CCN). However, a lack of genomic information has restricted studies on CCN resistance genes in Ae. variabilis and has limited genetic applications in wheat breeding.<h4>Results</h4>Using RNA-Seq technology, we generated a root transcriptome at a sequencing depth of 4.69 gigabases of Ae. variabilis No. 1 from a pooled RNA sample. The sample contained equal amounts of RNA extracted from CCN-infected and untreated control plants at three time-points. Using the Trinity method, nearly 52,081,238 high-quality trimmed reads were assembled into a non-redundant set of 118,064 unigenes with an average length of 500 bp and an N50 of 599 bp. The total assembly was 59.09 Mb of unique transcriptome sequences with avBMC genomics133https://www.ebi.ac.uk/biostudies/studies/S-EPMC3439707biostudies-literatureDe novo assembly and characterization of the root transcriptome of Aegilops variabilis during an interaction with the cereal cyst nematode.PMC3439707Pan ZFYu MQLong HZhang JXu DLLiang JJLi JLChen XDeng GBfalseDe novo assembly and characterization of the root transcriptome of Aegilops variabilis during an interaction with the cereal cyst nematode.<h4>Background</h4>Aegilops variabilis No.1 is highly resistant to cereal cyst nematode (CCN). However, a lack of genomic information has restricted studies on CCN resistance genes in Ae. variabilis and has limited genetic applications in wheat breeding.<h4>Results</h4>Using RNA-Seq technology, we generated a root transcriptome at a sequencing depth of 4.69 gigabases of Ae. variabilis No. 1 from a pooled RNA sample. The sample contained equal amounts of RNA extracted from CCN-infected and untreated control plants at three time-points. Using the Trinity method, nearly 52,081,238 high-quality trimmed reads were assembled into a non-redundant set of 118,064 unigenes with an average length of 500 bp and an N50 of 599 bp. The total assembly was 59.09 Mb of unique transcriptome sequences with av2012-01-01T00:00:00Z2012 Apr2026-04-29T13:53:11.077Z2026-04-07T15:15:32.285ZS-EPMC34397072249481410.1186/1471-2164-13-133